文档介绍：川芎嗪对缺氧缺血性脑损伤炎症细胞因子作用的实验研究
作者:吴洪华杨成石增立刘凤
【摘要】目的观察川芎嗪对新生鼠脑组织缺氧缺血性脑损伤(HIBD)后炎症细胞因子IL8、TNFα、IL10动态变化的影响。方法选用7日龄Wistar大鼠90只,随机分为假手术对照组、HIBD组、川芎嗪治疗组,检测0~48 h 5个不同时间点的效应指标的变化趋势及组间差异。结果 HIBD组各时间点IL8、TNFα、IL10浓度均比假手术对照组明显升高(P <001),川芎嗪治疗组IL8、TNFα、IL10浓度均比HIBD组降低。结论缺氧缺血早期促炎症细胞因子IL8、TNFα和抑炎症细胞因子 IL10表达上升,川芎嗪可减轻缺氧缺血脑损伤后新生鼠脑内炎症程度。
【关键词】川芎嗪;缺氧缺血性脑损伤;白细胞介素8;肿瘤坏死因子α;白细胞介素10
【Abstract】 Objective To study the effects of ligustrazine on the expression of IL8,TNFα and IL10 after hypoxicischemic brain damage in rats’ brain y Wistar rats of 7dayold were randomly divided into experiment group and control group. The enzymelinked immunosorbent assay was used to measure mean
concentrations of IL8,TNFα,IL10 in rats’ brain tissue after the model of HIBD was established. Results The mean concentrations of IL8,TNFα,IL10 in HIBD group were significant higher than those in sham operated group (P<001).In ligustrazine intervention group, compared with HIBD group, the concentrations were The concentrations of IL8,TNFα and IL10 were increased after HIBD, ligustrazine could regulate down the inflammation degree,which might be one of mechanisms of ligustrazine treating HIBD.
【Key words】 ligustrazine, hypoxicischemic brain damage,interleukin8,tumor necrosis factor alpha,interleukin10
缺氧缺血性脑损伤(hypoxicischemic brain damage, HIBD)是