肠内营养对梗阻性黄疸幼鼠肠黏膜Bax、Bcl2蛋白表达的影响及意义.doc

文档介绍：肠内营养对梗阻性黄疸幼鼠肠黏膜Bax、Bcl2蛋白表达的影响及意义
作者:胡凤爱张帆赵冬梅张璐萍刘烨辉傅廷亮
【摘要】目的探讨肠内营养对梗阻性黄疸幼鼠肠黏膜中Bax、Bcl2表达的影响及意义。方法将40只Wistar大鼠随机分为正常对照组、假手术组、梗阻性黄疸(OJ)组、OJ+能全素组,免疫组织化学法检测肠黏膜Bcl2和Bax蛋白的表达。结果 OJ组大鼠肠黏膜Bcl2表达降低,Bax表达升高,Bcl2/Bax比值下降(均P<);OJ+能全素组大鼠Bcl2表达增加(P<),Bax表达降低(P<),Bcl2/Bax比值升高(P<)。结论梗阻性黄疸时肠黏膜细胞凋亡显著增加,应用肠内营养对肠黏膜损伤有保护作用,其机制可能是与上调肠黏膜Bcl2蛋白及下调Bax蛋白有关。
【关键词】梗阻性黄疸;肠内营养;Bax;Bcl2
【Abstract】 Objective To study the effects of enteral nutrition on expression of Bax and Bcl2 in gut mucosa in rats with obstructive The Wistar rats were randomly divided into normal control group,sham operation group,obstructive jaundice(OJ) group,and obstructive jaundice group with nutrison(OJ+N).The expression of Bax and Bcl2 in intestinal mucosa was tested by immunohistochemical The expression of Bcl
2 in intestinal mucosa in OJ group decreased,while the expression of Bax increased,and the ratio of Bcl2/Bax decreased (P<,respectively),the expression of Bcl2 in OJ+N group increased (P<),while the Bax decreased (P<),and the ratio of Bcl2/Bax increased (P<).Conclusion Intestinal mucosa cell apoptosis increased significantly when obstructive jaundice nutrition helps lessen the intestinal mucosa damage caused by obstructive jaundice,with the increase of Bcl2 and the decrease of Bax in intestinal muc