文档介绍：金边瑞香活性成分瑞香黄烷-Ⅰ,瑞香素对SMMC-7721和 MCF-7细胞作用的研究
作者:舒奇,傅颖媛刘婷,余燕影
【摘要】目的研究金边瑞香活性成分瑞香黄烷-Ⅰ(E002)、瑞香素(E003)抗肿瘤活性,为进一步研究抗肿瘤机制提供依据。方法用噻唑蓝(MTT)法检测不同浓度E002、E003对SMMC-7721和MCF-7细胞的抑制率。结果①E002、E003浓度为250~ μg/ml时,作用SMMC-7721细胞,两者的抑制率为:(±)%~(±)%。②E002浓度为250~ μg/ml时,作用MCF-7细胞,其抑制率为:(±)%~(±)%。③E002对SMMC-7721和MCF-7细胞的IC50值分别为:(±)和(±) μg/ml;E003对SMMC-7721细胞的IC50值为:(±) μg/ml。结论金边瑞香活性成分瑞香黄烷-Ⅰ对SMMC-7721、MCF-7细胞,瑞香素对SMMC-7721细胞的增殖均有显著抑制作用,且呈现良好的剂量依赖性。
【关键词】金边瑞香; 抗肿瘤; 噻唑蓝法
Abstract:ObjectiveTo study the antitumor activity of Daphne odor extracts-E002 (daphnodorin I) and E003(in ) in vitro. MethodsMCF-7 and SMMC-7721 cells were treated respectively wit
h E002 and E003 at different concentrations, the growth inhibitory effects of E002, E003 were assessed using the MTT assay .Results(1)Both inhibition rate (%)were from ± to ± ,when SMMC-7721 cells were treated with E002, E003 in the concentration range of 250~(μg/ml).(2) Inhibition rate (%)were from ± to ±,when MCF-7 cells were treated with E002 in the concentration range of 250~ (μg/ml).(3)IC50 value E002 on SMMC-7721 and MCF-7 cells (μg / ml) were ± and ± ;IC50 value E003 on SMMC-7721 cells (μg / ml) was: ± . ConclusionDaphnodorin I has significant inhibitory effect on MCF-7 and SMMC-7721 cells in a do