文档介绍：麻杏止咳合剂的质量标准研究
【摘要】目的研究麻杏止咳合剂的质量控制方法。方法采用薄层色谱法对麻杏止咳合剂中麻黄、甘草、金银花进行定性鉴别,采用高效液相色谱法测定麻杏止咳合剂中盐酸麻黄碱的含量。结果麻杏止咳合剂中的麻黄、甘草、金银花的供试品薄层色谱中,在与对照品色谱相应位置上显相同颜色斑点; 0~ 0 μg范围内线性关系良好,%。结论该质控方法简便准确、灵敏度高,可以用于麻杏止咳合剂的质量控制。
【关键词】麻杏止咳合剂;盐酸麻黄碱;薄层色谱法;高效液相色谱法;质量标准
Abstract:Objective To study the quality control method of apricot kernel cough mixture. Methods Chinese ephedra, liquorice and honeysuckle in apricot kernel cough mixture were qualitative examined by thin-layered chromatography (TLC), and the content of ephedrine hydrochloride was measured by high performance liquid chromatography (HPLC). Results The spots in the TLC of Chinese ephedra, liquorice and honeysuckl were in the same color with those in the chormatograms of control articles. For ephedrine hydrochloride, linear range was 0~ 0 μg and the average recovery was %. Conclusion The quality control is simple, accurate and highly sensitive. The methods can be used for quality control of apricot k
ernel cough mixture.
Key words:apricot kernel cough mixture;ephedrine hydrochloride;TLC;HPLC;quality standard
麻杏止咳合剂是本院已故名老中医朱必泉的经验方,由麻杏石甘汤加味而来,具有辛凉解表、清热解毒、润肺止咳、化痰平喘作用。用于急性支气管炎、支气管肺炎,症见咳嗽痰盛、喘促气逆、咽痛等,特别对于风热型咳嗽疗效确切。本试验对其质量控制标准进行研究。
1 仪器与试药
HP1100高效液相色谱仪,包括UV可变波长检测器(美国惠普);Camag薄层自动涂布仪(日本岛津);UV-240紫外分光光度计(日本岛津),梅特勒-托利多Excellence XP205分析天平。盐酸麻黄碱对照品(批号110721-200512)、甘草对照药材(批号081-200423)、绿原酸对照品(