文档介绍：中药佳蓉片与雌激素对生殖系统作用的异同
作者:陈亚琼,杨海燕,黄艳红,吴曙光,叶雪清
【关键词】中成药
关键词: 中成药;卵巢切除术;子宫;小鼠;阴道
摘要:目的观察复方中药佳蓉片(JRP)与乙烯雌酚(DES)对生殖系统的不同作用. 方法用JRP低(JRP-L,6g kg-1 )、JRP中(JRP-M,12g kg-1 )、JRP高(JRP-H,24g kg-1 )三个不同剂量、DES( kg-1 )和生理盐水(Con-)每日分别灌胃处理70只未成年(17d)昆明小鼠3d和50只去卵巢成年(60d)昆明小鼠28d,成年小鼠实验中另设灌服生理盐水的假去卵巢组(Sham)和去卵巢组(OVX),观察对子宫质量/体质量百分比(PUM)、阴道上皮细胞角化百分比(VKEC)、血清17β雌二素(E2)水平的变化. 结果(1)PUM值JRP不同剂量处理未成年小鼠3d后,JRP-M组的PUM值高于Control组(,,P<),但明显低于DES组(,P<).JRP处理成年去卵巢小鼠28d后,OVX+JRP三个组的PUM值显著高于OVX组(,,,,P<),但明显低于OVX+DES组(,P<)和Sham组(<);(2)VKEC值OVX+JRP三个不同剂量组的VKEC值与OVX比较明显增加(,,,,P<),但增加值显著低于DES组(
,P<);(3)血清E2值OVX+JRP组和OVX+DES组的血清E2水平与OVX组比较无差异. 结论 JRP对小鼠子宫、阴道有弱雌激素样作用,但作用强度远低于DES,这种作用可能不是通过提高内源性E2水平而实现的.
Keywords:Chinese patent drugs;ovariectomy;uterus;mice;vagina
Abstract:AIM The investigated was designed to evaluate the different effects of diethylstilbestrol(DES)synthesized estrogen and Jiarongpian(JRP)made from traditional Chi-nese herbal medicines of tonifing the kidney,on reproductive system in female mature ovaritectomized(OVX)and imma-ture Kunming Fifty Kunming female im-mature(17d old)mice were dividing into five groups:JRP lower dosage(JRP-L,6g kg-1 d-1 ),JRP mediun dosage(JRP-M,12g kg-1 d-1 ),JRP higher dosage(JRP-H,24g kg-1 d-1 ),DES( kg-1 d-1 )and Control(normal saline NS, d-1 ),and were fed solution in-cluding JRP,DES or normal saline immature mice were sacrificed to examine the percentage of uterine mass(PUM).Sixty Kunming mature(60d old)female mice were divided into six groups:Sham operated(Sham, d-1 );OVX( d-1 );OVX+JRP-L(6g kg-1 d-1 ),OVX+JRP-M(12g kg-1 d-1 ),OVX+JRP-H(24g kg-1 d-1 ),OVX+DES( kg-1 d),and all mature mice were solution of JRP,DES or NS beginning3d after surgery of the animals were sacrificed to measure t