文档介绍：早期高压氧治疗对颅脑损伤神经细胞凋亡的影响
作者:刘勇,陈向林,朱玉辐,燕景峰,孔令胜,冯军,胡小兵
【摘要】目的通过研究早期高压氧治疗对大鼠颅脑损伤后相关基因Bcl-2、Bax表达影响,探讨其对损伤神经细胞的早期保护作用。方法清洁级SD大鼠100只,随机分为正常组(Nor)20只、创伤组(TBI)40只、高压氧治疗组(HBOT)40只。采用Allen′s改良法建立大鼠自由落体脑损伤模型,在损伤6 h内予以高压氧治疗。伤后8 h及1、3、5、8天处死动物取材,苏木精-伊红染色观察细胞形态学变化,流式细胞仪检测各观察点细胞凋亡率,免疫组织化学染色方法检测皮质及海马细胞中Bcl-2、Bax蛋白的表达。结果 HBOT组各时点皮质及海马细胞均较TBI组形态规则、排列整齐,而且层次较多,胞核浓染、固缩的现象明显减少。与TBI组相比,HBOT组各时点的细胞凋亡率均显著下降(P&lt;)。HBOT组各时点Bcl-2表达强度均较TBI组增强(P<,P&lt;),Bax表达强度均较TBI组显著降低(P<)。结论早期高压氧治疗能明显促进颅脑损伤后Bcl-2的高表达,抑制Bax的表达,能有效抑制颅脑损伤后细胞凋亡。
【关键词】脑损伤;早期;高压氧;Bcl-2;Bax;细胞凋亡
Abstract: Objective To investigate the effects of early hyperbaric oxygenation treatment (HBOT) on the expression of apoptosis genes Bcl-2 and Bax following the rat b
rain injury and study the neuronal protecting mechanism. Methods 100 Sprague-Dawley rats were divided into 3 groups: Normal group (20 rats), TBI group (40 rats), HBOT group (40 rats). Based upon the Feeney′s model of modified Allen′s method, experimental animals were treated with HBO within 6 hours. The animals were sacrificed at the 8th hour, and on days 1, 3, 5 and 8, respectively. Their hippocampus cells were observed for morphological changes by H-E staining; the apoptosis rate of injured hippocampus cortex was detected by flow cytometry; and the expression of protein Bcl-2 and Bax was detected with immunohistochemical staining. Results H-E staining indicated t