文档介绍：滋补肾阴方药对D-半乳糖拟衰小鼠自由基代谢及免疫功能的影响
【摘要】目的研究滋补肾阴方药对D-半乳糖(D-gal)所致衰老小鼠自由基代谢及免疫功能的影响。方法采用D-gal复制衰老小鼠模型。测定衰老小鼠血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,胸腺指数和脾指数,脾脏T淋巴细胞增殖活性及血清中白介素-2(IL-2)水平,并观察滋补肾阴方药对上述指标的影响。结果滋补肾阴方药能明显提高血清中SOD活性,降低血清中MDA含量,并提高胸腺指数、脾指数,脾脏T淋巴细胞增殖活性和血清中IL-2水平。结论抑制脂质过氧化反应,提高抗氧化酶活性,改善免疫功能可能是滋补肾阴方药延缓衰老的作用机制之一。
【关键词】中医药疗法衰老模型自由基免疫
Abstract:ObjectiveTo explore the effect of invigorating kidney-YIN recipe on the free radical metabolism and immune function of aging mice induced by D-(MDA) content and superoxide dismutase(SOD) activity in serum,thymus indexes, spleen indexes, activity of T lymphocyte and the level of IL-2 were determined in aging mice induced by D- effect of invigorating kidney-YIN recipe on the above indexes was invigorating kidney-YIN recipe could significantly increase SOD activity in serum; decrease MDA content in serum, enhance thymus indexes, spleen indexes, activity of T lymphocyte and serum IL-2 in
hibit lipid peroxidation,increase activity of antioxidase and improve immune function might be one of the mechanisms of invigorating kidney-YIN recipe against aging.
Key words:Therapeutics of traditional medicine; Aging model; Free radicals; Immune
自由基损伤和免疫功能低下是老化的重要机制之一。目前,中医延缓衰老研究以补肾占居绝对优势,温补肾阳延衰的研究已取得了一些进展[1],而滋补肾阴抗衰老的作用研究不多[2]。根据中医学阴阳互根,阴阳平衡的理论,补肾阴、补肾阳应该具有同等重要的作用。左归饮是中医滋补肾阴的代表名方,由熟地、