梅毒螺旋体IgM抗体检测的临床意义.doc

文档介绍：梅毒螺旋体IgM抗体检测的临床意义
作者:李步荣,贺军涛,张毅,岳天海,李丽华,张小玉
【Abstract】 AIM: To evaluate the clinical significance of detection of IgM antibody against Treponema pallidum in diagnosing syphilis. METHODS: The serum samples of 90 syphilitic patients were collected and treated with IgG/RF immunoabsorbent, then were detected for TPIgM with Treponema pallidum particle agglutination(TPPA) assay. The serum samples were also detected for TPDNA by RTPCR method. RESULTS: The TPIgM positive rates were %, 100% and % in primary syphilis, secondary syphilis and latent syphilis, respectively. The total positive rate of TPIgM was %(73/90) in syphilis sera. The positive rates were statistically different between the 3 different syphilis groups(χ2=, υ=2, P<). The TPDNA positive rate was %(71/90). There was no significant difference between the positive rates of TPIgM and TPDNA(χ2=, υ=1, P>). CONCLUSION: The detection of IgM antibody against Treponema pallidum in serum is important for the clinical diagnosis of syphilis.
【Keywords】 syphilis; IgM antibodies; treponema pallidum
【摘要】目的: 评价检测梅毒螺旋体IgM型抗体的临床意义. 方法: 收集经临床确诊为梅毒患者的血清标本90例,用RTPCR方法进行TPDNA检测,用IgG/RF吸收剂处理血清,然后应用梅毒螺旋体明胶凝集试验进行TPIgM的检测. 结果: 一期梅毒的 TP%,二期梅毒的阳性率100%,隐性梅毒的阳性率 %. %(73/90). TPIgM在不同时期梅毒组中检测阳性率差异有统计学意义(χ2=, υ=2, P<). TP%(71/90). TPDNA和TPI