文档介绍：蛤蟆油紫外谱线组法真伪鉴别的研究
作者:王永生, 姜大成, 白雪媛, 王恩思
【关键词】蛤蟆油;,,紫外谱线组法;,,鉴别
摘要:目的利用紫外谱线组法对蛤蟆油进行检测,绘制出蛤蟆油的紫外谱线组图谱。方法对蛤蟆油进行石油醚、三***甲烷、醋酸乙酯、正丁醇、95%乙醇超声波提取后,再进行相应的紫外及其一阶导数光谱检测。结果蛤蟆油的紫外谱线组图谱重现性较好。结论通过紫外谱线组法,可以绘制出蛤蟆油的紫外谱线组图谱,达到蛤蟆油真伪鉴别的目的。
关键词:蛤蟆油; 紫外谱线组法; 鉴别
Abstract:ObjectiveUASLG was used for Rana temporatia chensisnensis David's identification. UASLG's action spectrums for Rana temporatia chensinensis David was described in this paper for the first. Methods Rana temporatia chensinensis David were distilled with method of ultrasonic in petroleum ether、chlorofomn、ethyl acetate、n-butanol and ethanol (95%), then their absorbance in UV and one differential coefficient spectrum were determined. Results Rana temporatia chensinensis David's UASLG has good reappearance. Conclusion Rana temporatia chensinensis David can identified by UASLG.
Key words:Rana temporatia chensinensis David; UASLG; Identification
蛤蟆油(Oviductus Ranae)为珍贵动物性中药材,据《中国药典》(2005年版)Ⅰ部收载,为蛙科动物中国林蛙 Rana temporaria chensinensis David雌蛙的输卵管,经采制干燥而得。具有补肾益精、养阴润肺的功效。临床多用于阴虚体弱,神疲乏力,心悸失眠,盗汗不止,痨嗽咳血[1]。国内蛤蟆油的使用较为混乱,由于正品蛤蟆油价格较贵,市场上常有以伪品蛤蟆油充当正品蛤蟆油销售使用的情况,其外观性状较为相似,尤其是蛤蟆油常粉成细粉后调剂,伪品蛤蟆油更易于正品蛤蟆油混淆,误用、混用现象时有发生。为了寻找快速、简便、准确地鉴别正品蛤蟆油与伪品蛤蟆油,特别是鉴别粉末的方法,我们对正品蛤蟆油和伪品蛤蟆油的石油醚、三***甲烷、醋酸乙酯、正丁醇和95%乙醇提取液分别进行了紫外光谱法鉴别研究。本文用紫外谱线组法对其进行鉴别,此方法鉴别中药材具有特征性强、取样量小、简便、快速、准确等特点且方法可靠,简单,重现性强,可供鉴别参考[2,3]。现将结果报道如下。
1 材料
安捷伦 UV8453型分光光度计,石油醚、三***甲烷、醋酸乙酯、正丁醇和95%乙醇均为分析