文档介绍：人参皂苷Rg2对体外培养低氧心肌细胞的保护作用
【摘要】目的观察人参皂苷Rg2对低氧心肌细胞的保护作用。方法取新生24 h的Wistar大鼠心肌细胞,体外培养4 d,随机分为正常组、模型组、人参皂苷Rg2组。 mmol/L的人参皂苷Rg2,正常组与模型组加入等量的培养液。孵育4 h后,将模型组与人参皂苷Rg2组移入37 ℃恒温密闭容器中,,流速20 mL/min,持续1 h,然后应用锥虫蓝染色计数存活细胞,流式细胞仪检测细胞凋亡情况,酶法测定超氧化物歧化酶(SOD)的活性及丙二醛(MDA)、一氧化氮(NO)的含量。结果人参皂苷Rg2组与正常组心肌细胞凋亡率比模型组明显降低(F=,q=~,P<);人参皂苷Rg2组与正常组心肌细胞SOD活性、MDA和NO含量与模型组相比差异有显著性(F=~,q=~,P<)。结论人参皂苷Rg2在体外有抗低氧损伤的作用,其作用机制可能是通过抑制细胞凋亡、提高抗氧化酶的活力、清除自由基而发挥作用。
【关键词】人参皂苷;肌细胞, 心脏;细胞培养技术;低氧;自由基
[ABSTRACT] Objective To investigate the protection of Rg2 on hypoxic cardiocytes. Methods Cardiocytes of 24 hour old newborn rat were cultured for four days then divided into normal group, model group and Rg2 group. Rg2 ( mmol/L) was added to Rg2 group, and culture fluid was added to the normal group and model group. All were incubated for four hours, and then model group and Rg2 group were taken to 37
℃ homeothermic hermetic container, and given 95% N2 and 5% CO2 and mixed. The morphological changes were observed. The apoptosis of cardiocytes were detected by flow cytometry (FCM). The contents of MDA and NO and the activity of SOD were determined by biochemical method. pared with the model group, the apoptosis significantly decreased in the Rg2 and the normal groups (F=,q=-,P<). Compared with the model group, the act