文档介绍：心舒滴丸中人参提取、精制工艺研究
【摘要】目的优选心舒滴丸中人参提取、精制工艺条件。方法以人参皂苷Rg1、Re含量为指标,采用正交试验设计,优选人参提取工艺条件;以比吸附率、洗脱量为指标优选大孔吸附树脂精制工艺条件。结果优选提取工艺条件:体积分数为70%的乙醇6倍量,提取3次,每次1 h;精制纯化工艺条件:采用AB8型大孔吸附树脂,以体积分数为70%的乙醇为洗脱溶剂,4倍树脂体积。结论优选工艺可较好的提取纯化人参皂苷有效成分。
【关键词】心舒滴丸;提取工艺;人参皂苷Rg1;人参皂苷Re;大孔吸附树脂
Abstract:Objective To optimize the extraction procedure of ginenoside from Panax ginseng C for the quality control of Xinshu dripping pills,a traditional Chinese By measuring the concentration of ginenoside Rg1,ginenoside Re in the fractions with HPLC,the extraction process was evaluated by orthogonal purification process was evaluated by optimizing the adsorption and desorption rate of macroporous The optimum extraction conditions as follows: 6 volume times of 70% ethanol,3 times of extraction,1 hour of extraction time. AB
8 macroporous resin was selected,and eluant was 70% of The method is effective for largescale extraction and purification of ginsenosides from Panax ginseng C.
Keywords:Xinshu dropping pills; extraction process;ginenoside Rg1;ginenoside Re;macroporous adsorption resin
心舒滴丸由人参、丹参、冰片等药物组成,用于冠心病的治疗,人参为方中主药,人参皂苷为其主要活性成分之一,具有明显的扩张冠状动脉、增加心肌血流量、提高心肌耐缺氧能力、调节心律和血压等作用。本文采用高效液相色谱法,以人参皂苷Rg1、Re的含量为指标[1,2],优选人参最佳提取工艺及大孔吸附树脂精制工艺条件,为生产提供依据。
1 仪器与试药
DIONEX 3000高效液相色谱仪(在线脱气、自动进样、光电二极管阵列检测器、Chromeleon色谱工作站,美国戴安公司);人参皂苷 Rg1对照品(批号:110754-200320,供含量