文档介绍：我国哈萨克族与汉族维生素D受体基因多态性的分布
作者:陈瑞英,冷兴文,阿丽亚,李红,蒋银花,陶国枢,张红红,刘建伟,高宇红
【关键词】受体
关键词:受体,维生素D;基因;聚合酶链反应;骨质疏松中图号: 文献标识码:A
摘要:目的为研究我国哈萨克族与汉族维生素D受体基因多态性分布与骨质疏松的关系. 方法采用聚合酶链反应(PCR)限制性片段长度多态性技术,对哈萨克族(年龄50±2岁)的健康妇女93例,和汉族(年龄59±3岁)健康妇女179例进行VDR基因检测. 结果在中国哈萨克族93例中,bb型44例(47%),Bb型45例(48%),BB型4例(4%);在汉族179例中,bb型162例(90%),Bb型17例(10%),未见BB型. 结论中国哈萨克族VDR基因多态性分布与汉族相比存在非常显著性差异(P<).
Keywords:receptor,vitamin D;genes;polymerase chain reac-tion;osteoporosis
Abstract:AIM To explore the difference between Kazakh and the Han in the distribution of vitamin D receptor(VDR)gene VDR genotypes in179health postmenopausal women of the Han nationality(age:59±3years),and in93those of the Kazakh(age:50±2years),and also in17cases of osteoporosis patients were ana-lyzes by polymerase chain reaction restriction fragment length Frequencies of bb,Bb and BB genotype are90%,10%and0%in the the Han,and47%,48%and4%in the Kazakh significant differ-ence is found in the frequency distribution of VDR genotype between the Han and the Kazakh(P<).CONCLU-SION Han and Kazakh differ in the frequency distribution of VDR genotype.
0 引言
维生素D受体(vitamin D receptor VDR)基因多态性,由位于12q 13 ,Morrison等[1] 研究报告,VDR基因在内切酶BsmⅠ,ApaⅠ,TagⅠ作用下,(bone mineral density,BMD)