蒲葵子的体外抗癌活性.doc

文档介绍：蒲葵子的体外抗癌活性
作者:陈艳, 林新华, 李少光, 翁绳美, 姚宏
【摘要】目的研究蒲葵子提取物的各萃取部位体外抗肿瘤作用。方法采用四甲基偶氮唑盐(MTT法)比色法,观察蒲葵子提取物的各萃取部位对肝癌细胞HepG2和白血病细胞HL60增殖的抑制作用。结果蒲葵子提取物的各萃取部位在体外对肝癌细胞HepG2和白血病细胞HL60有明显的抑制作用,,效果最为显著。结论蒲葵子提取物的各萃取部位能抑制肝癌细胞HepG2和白血病细胞HL60的增殖,有较强的体外抗肿瘤活性。
【关键词】棕榈科; 抗肿瘤药; 提取法
ABSTRACT: Objective To investigate anti-tumor effect of the extracts of Livistona chinesis in vitro. Methods The cytotoxic effects of the four kind extracts of Livistona chinesis on two tumor cell lines cultured in vitro were tested by MTT assay. The tumor cell lines were HepG2 and HL60. Results The extracts of Livistona chinesis induced significant growth arrested in two malignant tumor cell lines which cultured in vitro. IC50 of these cells exposed to the extracts from N-butanol for 48 hours approach % and %. Conclusion The N-butanol extracts of Livistona chinesis had obvious anti-tumor activity in vitro.
KEY WORDS: Palmaceae; antineoplastic agents; extraction
蒲葵(Livistona chinesis .)属于棕榈科(Trachycar Pus Fortinei)常绿乔木,蒲葵子为该植物种子,其性味平、淡,具有败毒抗癌、消瘀止血之功效。民间常用其治疗白血病、鼻咽癌、绒毛膜癌、食管癌,效果显著[1-2]。笔者对福建产蒲葵子进行初步提取和分离,并研究各提取部位体外抗肿瘤活性,筛选有抗癌活性的提取部位。
1 材料与方法
材料
试剂
全自动酶标仪(Mod550,美国Bio-Rad公司);二氧化碳培养箱(美国Forma公司);RPMI 1640培养基(美国Gibco公司);小牛血清(美国Hyclone公司);胎牛血清(澳大利亚Maverick公司);四甲基偶氮唑盐(MTT,美国Amresco);胰蛋白酶(美国Difco公司);噻唑蓝(华美生物工程公司北京分公司);其余试剂均为国产分析纯。
药物
采集福建福州地区产蒲葵子,经福建医科大学天然药物学系张永