文档介绍：Cazymes 对比分析流程NCBI 数据库地址 .gov/以 Fibrobacter inogenes 为例。 NCBI 数据库,输入 Fibrobacter inogenes,在 Gene 下查找,图 1进入下面的界面,图 2以 FSU_2293 为例,基因注释为木聚糖酶,点击进去,图 3发现 FSU_2293,属于 GH10,有 CBM6,将此基因进行统计,见下表。GH10 FSU_2293 CBM6 BTD xylanase xylanase若是 NCBI 上没有显示相关的 GH 家族或 CBM,则可以进行 BLAST。点击图3 中的“FASTA”,复制相关基因进行 BLAST。例如 CHU_0868,是糖基转移酶相关的基因,但是没有显示具体的 GT 家族。图 4进行 BLAST,图 5点击相关的结构域,进入另一个网页,图 6可以看到,此基因属于 GT1 家族。或是进行 Pfam 结构域搜索:网址:http://pfam./search,输入相关基因,点击 ,图 7可以看到,此基因具有 GT1 结构域,属于 GT1 家族。CAZyFamilyMYP LocusCBMFamilyIn SilicoPredictionCharacterized ActivityGH(74)GHMYP_0567hypothetical proteinGHMYP_0581hypothetical proteinGHMYP_0913hypothetical proteinGHMYP_1026hypothetical proteinGHMYP_1096hypothetical proteinGHMYP_3811endoglucanaseendoglucanaseGHMYP_4017xyloglucanasexyloglucanaseGHMYP_4345CBM9beta-xylosidase/endoglucanasebeta-xylosidase/endoglucanaseGHMYP_4348CBM4_9glycerol kinaseglycerol kinaseGH1MYP_2015beta-galactosidasebeta-galactosidaseGH2MYP_3616beta-mannosidasebeta-mannosidaseGH3MYP_0984b-glucosidaseb-glucosidaseGH3MYP_1602hypothetical proteinGH3MYP_1909b-glucosidaseb-glucosidaseGH3MYP_2588xylosidase/arabinosidasexylosidase/arabinosidase“Descriprition”。分别统计 glycoside hydrolase(GH)、glycosyl transferase(GT)、 haridelyase(PL)、carbohydrate Esterase(CE)。对于 glycoside hydrolase 可以统计与纤维素降解和半纤维素降解相关的基因。格式见下表。注意:表中标红的列为必填项目,“Characterized Activity”为图 2 中的GH3MYP_3067glycoside hydrolaseGH3MYP_4245xyl