文档介绍：
利用 DNA 条形码技术鉴定北京延庆松山地
区舟蛾科方法的研究#
于晓溪1,周晓雪1,杨聪慧1,武春生2,张爱兵1**
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(1. 首都师范大学生命科学学院,北京 100048;
2. 中国科学院动物研究所动物进化与系统学重点实验室,北京 100101)
摘要:目的:本研究采用系统进化的方法鉴定北京延庆松山地区的舟蛾科鳞翅目昆虫。方法:
提取蛾类样本 DNA,对 COI 基因进行扩增和测序。分别利用建立系统进化树的方法,诊断
特征位点的方法以及通用混合 Yule 溯祖模型(general mixed Yule-coalescent,GMYC)的方法,
对舟蛾科进行聚类。结果:本研究结果表明这三种方法都能较好的将其区分归类。
关键词:舟蛾科;DNA 条形码;系统发育;诊断特征位点;GMYC
中图分类号:
Species identification of moth species (Notodontidae) using
DNA barcoding
YU Xiaoxi1, ZHOU Xiaoxue1, YANG Conghui1, WU Chunsheng2, ZHANG Aibing1
(1. College of Life Sciences, Capital Normal University, Beijing 100048;
2. Key Laboratory of Zoological Systematics and Evolution, Chinese Academy of Sciences,
Beijing 100101)
Abstract: Objective: To identify moth species (Notodontidae) from Songshan,Yanqing,and
Beijing using DNA barcoding. Method: Total genomic DNA was extracted from specimens of
moths. Mitochondrial COI sequences were amplified. Species identifications were performed
using neighbor-joining (NJ), diagnostic characters and general mixed Yule-coalescent(GMYC)
methods. Conclusion: DNA barcoding produces good classification results and all these methods
could classify these species correctly.
Key words: Notodontidae; DNA barcoding; ic tree; diagnostic character; general
mixed Yule-coalescent
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0 引言
松山是北京市首个国家级自然保护区,以松得名,该自然保护区内有华北地区唯一的天
然次生油松林。地理坐标为东经 115°43′44″-115