文档介绍：
基于分子标记选育硬粒小麦-长穗偃麦草 E
染色体附加系#
黄泽峰1,曹文广2,刘惠萍1,戴毅1,高勇1,DawnChi2,e Fedak2,陈建
5
10
15
民1**
(1. 扬州大学生物科学与技术学院;
2. 加拿大东部谷物和油料作物研究中心)
摘要:以硬粒小麦 Langdon (Triticum durum)与含有长穗偃麦草 E 基因组的异源六倍体小麦
品系 8801 (AABBEE)杂交衍生的后代为材料,利用细胞学和分子标记技术结合检测跟踪长
穗偃麦草染色体,选育硬粒小麦-长穗偃麦草双体异附加系。对不同世代通过染色体数目检
查为 2n=29 或 30 的植株提取 DNA,利用长穗偃麦草 1E-7E 染色体特异分子标记对这些植株
DNA 进行 PCR 扩增,最后 F4 代的 PCR 扩增结果表明 1 株含有长穗偃麦草 3E 染色体,2
株含有 4E 染色体,2 株含有 5E 染色体, 2 株含有 7E 染色体,而且具有 7E 染色体植株的
染色体数目分别为 29 和 30。初步确定为硬粒小麦-长穗偃麦草 7E 单体和双体附加系。讨论
了有关创建附加系的途径。主要研究成果,字数在 200~400 字左右为宜,不用第一人称做
主语,不与正文语句重复。
关键词:长穗偃麦草;硬粒小麦;附加系;染色体特异分子标记
中图分类号:Q37
20
Development of Triticum durum- Thinopyrum elongatum E
chromosome addition lines based on chromosome specific
markers
HUANG Zefeng1, CAO Wenguang2, LIU Huiping1, DAI Yi1, GAO Yong1, Dawn
25
30
35
40
45
Chi2, e Fedak2, CHEN Jianmin1
(1. College of Bioscience and Biotechnology, Yangzhou University;
2. Eastern cereal and oilseed research center, Ottawa, ON. Canada)
Abstract: IThinopyrum elongatum, tall wheatgrass, is a grass species with many excellent traits
that can be used mon wheat improvement. Objective of this study was to develop Triticum
durum- Thinopyrum elongatum E chromosome addition lines. A durum wheat cultivar “Langdon”
(Triticum durum, AABB) was crossed with the partial hexaploid amphiploid line 8801 with
genome AABBEE. F1 plants were obtained through embryo rescue. The progeny of F2, F3 and F4
were analyzed for development of T. durum-Th. elongatum alien addition lines based on
combined techniques of cytology and molecular marker selection to track exogenous
chromosomes. DNAs of plants with 29 or 30 chromosomes were isolated from leaf tissue at each
generation. PCR was conducted for chromosome-specific molecular markers of Th. elongatum
to identify specific additional lines. The results showed that in F4 progeny, six plants had 29
chromosomes: one plant containing 3E chromosome; two plants containing 4E chromosome; two
plants containing 5E