文档介绍：
拟薄水铝石/海藻酸复合凝胶固定化纤维素
酶
巩桂芬,朱丽娜,吴谙宇*
5
10
(哈尔滨理工大学材料科学与工程学院,哈尔滨 150040)
摘要:以拟薄水铝石和海藻酸钠为原料,合成复合凝胶,并以此复合凝胶为固定化纤维素酶
的载体。以海藻酸固定化纤维素酶的包埋率为 60%,而以复合凝胶固定化纤维素酶的包埋率
超过了 %。探讨了底物 CMC 在复合水凝胶材料中的有效扩散系数,表明当拟薄水铝石
的掺杂量为 %时,有效扩散系数最大。讨论了 pH、温度对固定化酶和游离酶活力的影
响,结果表明固定化酶具有对 pH 和温度更好的稳定性。固定化酶的可重复实用性和储藏稳
定性良好,连续使用 10 次后酶活力保留 46%,储藏 30 天后固定化酶依旧有 61%的酶活力。
关键词:材料学;拟薄水铝石;海藻酸钠;固定化
中图分类号:
15
Cellulase immobilized by pseudo-boehmite/alginate
composite gel
GONG Guifen, ZHU Lina, WU Anyu
(School of Material Science & Engineering, Harbin University of Science and Technology, Harbin
20
25
30
35
40
150040)
Abstract: Pseudo-boehmite was essfully introduced into alginate to posite gels.
When the pseudo-boehmite addition is %, posite gel has the maximum effective
diffusion coefficient of CMC. Using the gel as a carrier, the enzyme loading efficiency, enzymatic
activity, and the stability of immobilized cellulase were investigated. Loading efficiency of
cellulase in ALG gel was 60%, and the loading efficiency in α-AlOOH/posite gel
increased significantly, with the highest loading efficiency reaching to %. Comparing the free
cellulase and immobilized cellulase, the latter have higher thermostability and pH-stability. The
immobilized cellulase has significant reusability and storage stability for extended periods of time.
In the batch reaction, the immobilized c