文档介绍：Journal of Plant Physiology and Molecular Biology 2005, 31 (5): 477-484 477
Cloning, Characterization and Expression of Wheat EDR1 (Enhanced
Disease Resistance) Gene
NIU Ji-Shan 1*, ZHANG Li-Na2, HONG De-Feng3, WANG Ying-Hong1
(1National Center of Engineering and Technological Research for Wheat, Henan Agricultural University, Zhengzhou 450002, China; 2College
of Life Sciences, Northwest Normal University, Lanzhou 730070, China; 3College of Agriculture, Xinjiang Agricultural University, Urumchi
830052, China)
Abstract: To investigate if there is an EDR1 pathway that mitogen-activated protein kinase (MAPK) cas-
in wheat (Triticum aestivum L.), a pair of degenerate cades play important roles in elicitor signal transduc-
primers was designed according to the cDNAs of tion in plants (Hirt 2000; Ren et al. 2002; Chinnusamy
Arabidopsis thaliana EDR1 gene and its homologs et al. 2004). Recent studies suggest that o MAPK
were used to isolate EDR1 gene homologs from wheat. cascades including SIPK (salicylic acid-induced pro-
RT-PCR was conducted on the cDNA template syn- tein kinase), WIPK (wound-induced protein kinase),
thesized with RNA of wheat leaves. A 627-bp cDNA and NtMEK2 (Nicotiana tabacum MEK2, a o
fragment representing an EDR1 gene (named as MAPKK), an upstream kinase for both SIPK and
TaEDR1) was isolated (GenBank accession number: WIPK, have a crucial functio