文档介绍：11 1
Exploitation of Structural and Functional Genomics Databases for Gene
Identification in Peach
. Zhebentyayeva, D. Jiwan, . Jun, . Lalli, . Reighard
S. Forrest, J. Duncan, D. Main and . Abbott Department of Horticulture
Department of ics & Biochemistry Clemson University
Clemson University Clemson, SC 29634
Clemson, SC 29634 USA
USA
A. Callahan and R. Scorza
USDA-ARS Appalachian Fruit Research Station
Kearneysville, WV 25430
USA
Keywords: Prunuspersica, physical map. BAC sequencing, EST-SSR markers
Abstract
This research aims at the development of peach as a model genome for the
identification, characterization, and cloning of important genes in Rosaceae species.
We have constructed an initial physical map for peach. The map is anchored onto the
general Primus ic map and has been integrated with a peach transcript map
(). Our efforts to exploit the peach genomic databases are
focused on two genomic regions of interest: the distal part of linkage group 2 asso-
ciated with the genes for root-knot nematode resistance and the distal part of linkage
group 4 which is important for fruit characters such as freestone and melting flesh.
Our bines linkage ic mapping using EST-based ic markers,
localization of the markers on the physical framework, and sequencing the genomic
region of interest. Preliminary results illustrate the efficien