文档介绍：
Cloning and expression of a peroxiredoxin gene from
Capsella bursa-pastoris#
TAO Peng, HUANG Xiaojun, WANG Jianbo
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(College of Life Sciences, Wuhan University, Wuhan, 430072, China)
Abstract: In this study, an antioxidant gene was isolated and cloned from the widespread plant
Capsella bursa-pastoris. The antioxidant gene was homologous highly to the Arabidopsis ATPER1
gene, and was named as CbPER1. We deduced the amino acid sequence of CbPER1 protein, and used
it to conduct a ic bining with 1-Cys Prxs (1-cysteine peroxiredoxins) from other
plants for estimating the relationships. Using RT-PCR, we analyzed the expression of CbPER1 in
ans and embryonic stages. CbPER1 was extensively expressed in seeds, especially middle
and later seeds. Besides, CbPER1 rarely responded to heat stress, cold stress, and ABA treatment in
young leaves. Similarly, CbPER1 was not induced to express in young flowers when C. bursa-pastoris
plants were treated with ABA. CbPER1 showed down-regulation in dormant embryos after GA
treatment. Furthermore, the transcriptional level of CbPER1 in C. bursa-pastoris pared to that
of the Arabidopsis AtPER1, and both shared similar gene expression profiles. Our findings suggest that
the CbPER1 genes play significant roles in seed maturation, dormancy, and germination, not in the
response to abiotic stresses in C.