文档介绍：
水稻粉质突变体 OsPDIL1-1 的基因定位和
候选基因分析
韩小花,陈红,江玲,王益华,郭洪年**
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(南京农业大学作物遗传与种质创新国家重点实验室,南京 210095)
摘要:谷类作物种子内淀粉的积累对于作物产量的形成具有极为重要的意义。种子淀粉的生
物合成是各种酶催化的过程,这一模型已经初步建立。然而随着越来越多的该模型外的粉质
突变体基因的发现,新的多网络交叉模型的建立显得尤为重要。本研究将筛选到的粉质胚乳
突变体 T3612 与 NJ11 构建群体,利用 F2 群体中的 1242 个粉质表型极端个体将 T3612 突变
基因定位于第 11 染色体 BAC 克隆 OSJNBa0058P12 的 61 kb 区间。经 RICEGAAS 预测该区
间有 11 个基因,其中 PDIL1-1 基因发生突变。基因组序列测序结果显示 Protein Disulfide
Isomerase Like 1-1 (PDIL1-1)基因从 ATG 后 123 bp 处开始缺失,共缺失 4142 bp。扫描电镜
观察显示突变体 T3612 中的淀粉颗粒变圆、变小,淀粉合成相关酶活性检测发现 Pho1 以及
PUL 活性显著下降,而 SSI 活性显著上升。因此我们认为 PDIL1-1 的缺失显著影响淀粉合
成相关酶活性,而这极可能是影响淀粉合成的主要原因。
关键词:水稻;胚乳;粉质;淀粉合成;精细定位
中图分类号:S511
Gene Mapping and candidate gene analysis of a Floury in
rice (Oryza sativa L.)
HAN Xiaohua, CHEN Hong, JIANG Ling, WANG Yihua, GUO Hongnian
(Nanjing Agricultural University, National Laboratry of Crop ics and Germplasm
Enhancement, NanJing 210095)
Abstract: Starch accretion determines final grain weight and thereby it contributes greatly to grain
productivity. The enzymatic process model required for starch synthesis has been established by a
series of mutants using maize and rice as a anism. However studies on some new opaque
endosperm mutants showed that these new genes can not be added into the old model which has been
established, so we need construct a bigger or a cross model using these novel mutants. To ically
map the mutation we developed an F2 population derived from a cross between T3612 which is an
indica variety, and japonica variety NJ11. Furthermore, we used 1242 floury segregants to locate
within a 61 kb region of BAC OSJNBa0058P12 which located on chromosome 11. RiceGAAS
(/) predicted eleven ORFs in this map region. The mutation gene was
Protein Disulfide Isomerase Like 1-1 (PDIL1-1). DNA-PCR amplification and sequencing revealed a
4142bp deletion started from 123bp in PDIL1-1 CDS. Scanning electron microscopic examination
revealed that the mutant T361