文档介绍：Inhibitory effect of bined with 1-MTon subcutaneous xenografts of gastric cancer in mice【ABSTRACT】OBJECTIVE: To observe the inhibitory effect of FOLFOX4 combined with 1-MT on subcutaneous xenografts of gastric cancer in mice,and its influence on the expression of indoleamine-2,3-dioxygenase (IDO) in gastric cancer tissue. METHODS:Transfect the IDO eukaryotic expression plasmids (-IDO) into mice gastric cancer cell line MFC. The cell line expressing IDO stably was established. Expression of IDO was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Suspension of the MFC cells transfected with -IDO plasmid were inoculated subcutaneously in mice. Mice gastric carcinoma subcutaneous xenografts model expressing IDO highly was established (n=32). Blank control (n=8,untransfected MFC cells) and negative control (n=8,transfected MFC cells) were set up. The 32 model mice were randomly divided into 1-MT treatment group, FOLFOX4 treatment group,FOLFOX4+1-MT combined treatment(saline) group and non-treatment group (positive 【摘要】 目的: 观察FOLFOX4联合1-甲基-色氨酸 (1-methyl tryptophan,1-MT)对荷胃癌小鼠皮下移植瘤生长的抑制作用,以及对胃癌组织吲哚胺-2,3-双加氧酶 (indoleamine-2,3-dioxygenase,IDO)表达的影响。方法:将IDO真核表达质粒 (pcD