HPLC 法测定夏枯草口服液中迷迭香酸的含量.pdf

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— 1010 —药物分析杂志 Chin J Pharm Anal 2009, 29 (6)
HPLC法测定夏枯草口服液中迷迭香酸的含量
靳如义,安万学,潘相华,赵虹,任明太
(贵阳新天药业股份有限公司,贵阳 550018)
摘要目的:建立高效液相色谱法测定夏枯草口服液中迷迭香酸的含量。方法:采用 Phenomene C18柱( 250 mm × 4 6 mm, 5
μm) ,甲醇- 1%甲酸(40∶60)为流动相,流速: 1 0 mL· m in - 1 ,检测波长 330 nm,柱温: 25 ℃,进样量为 10μL。结果:迷迭香
酸在 18~450μg· mL - 1浓度范围内呈线性关系( r = 0 9999) ;平均回收率为 97 2% , RSD = 1 3% ( n = 6) 。结论:本法准确、简
便,可用于测定夏枯草口服液中迷迭香酸的含量。
关键词:高效液相色谱法;夏枯草口服液;迷迭香酸;含量测定
中图分类号: R917 文献标识码: A 文章编1 号: 0254 - 1793 (2009) 06 - 1010 - 03
HPLC determ ination of rosmarin ic acid in Xiakucao oral solution
J IN Ru - yi, AN W an - xue, PAN Xiang - hua, ZHAO Hong, REN M ing - tai
( Gui Yang Xin Tian Pharmaceutical Co. , L td, Guiyang 550018, China)
Abstract O bjective: To develop an HPLC method for determ ination of rosmarinic acid in Xiakucao oral solution.
M ethods: The samp le was separated on a Phenomene C18 (250 mm × 4 6 mm , 5μm) column with methanol - 1%
form ic Acid (40∶60) as mobile phase. The flow rate was 1 0 mL · m in - 1 , and the detection wavelength was set at
330 nm. The column temperature was 25 ℃. The injection volume was 10μL. Results:W ithin the range of 18 - 450
μg· mL - 1 , rosmarinic acid p resented a fine linear relationship between the injectedamounts and peak areas( r =
0 9999) ; The average recovery was 97 2% with RSD of 1 3% ( n = 6). Conclusion: The p rop