SSR标记研究粉花石斛的遗传多样性.doc

文档介绍：
SSR 标记研究粉花石斛的遗传多样性#
蔡小彦,冯震宇,侯北伟,丁小余**
(南京师范大学生命科学学院,南京 210023)
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摘要:本论文利用磁珠富集法构建粉花石斛微卫星文库,筛选出有效 SSR 标记,共获得 12
对多态性引物。通过对 9 个粉花石斛居群的 95 个个体的扩增发现:每个位点的等位基因数
目(A)为 3-22 个,平均每个位点 个等位基因;多态信息含量(PIC)为 -,
平均多态信息含量是 ;期望杂合度(He)为 -,平均期望杂合度是 。
利用开发的微卫星位点对粉花石斛的遗传多样性和居群遗传结构进行进一步分析。每个居群
检测到的等位基因数目是 -,每个居群的期望杂合度(He)是 -,平均期
望杂合度是 。结果亦表明粉花石斛具有较高的遗传多样性。AMOVA 分析表明居群内
的遗传变异占主要部分(%),居群间的变异占总变异的 %。聚类分析表明 9 个
野生居群的 96 个个体分为两大类,且居群间的遗传距离和实际的地理距离没有明显相关性。
关键词:植物;粉花石斛;SSR;遗传多样性
中图分类号:Q941
Development of microsatellite markers for ic diversity
analysis of Dendrobium loddigesii Rolfe
Cai Xiaoyan, Feng Zhenyu, Hou Beiwei, Ding Xiaoyu
(College of Life Sciences, Nanjing Normal University, NanJing 210023)
Abstract: New microsatellite DNA was isolated from D. loddigesii genome DNA through the
methods of entichment by ic beads. Twelve polymorphic microsatellites were development
for D. loddigesii, and their polymorphism was tested across all individuals from nine natural
populations. A total of 98 alleles were detected with an average of alleles per locus. The
average expected heterozygosity and polymorphism information content values were and
, respectively. Lastly, twelve novel microsatellite markers were employed to investigate the
ic diversity and population ic structure o