文档介绍：
Evaluation of ic Diversity of 48 Potato Onion (Allium
cepa var. aggregatum Don.) Germplasms Using SSR and
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AFLP markers#
JIN Xue, WU Fengzhi**
(Northeast Agricultural University, Mucai 59, Xiangfang, Harbin 150030, . China)
Abstract: Simple sequence repeat (SSR) markers and amplified fragment length polymorphism (AFLP)
were used to detect the ic diversity of 48 potato onion germplasms from Heilongjiang and Jilin
provinces in China. Twenty three SSR primers with rich polymorphism, stable amplification and good
repeatability were screened from one hundred and one SSR primers. With these screened primers, 116
bands were identified, of which 66 bands were polymorphic. The ic similarity was estimated with
the Nei-Li coefficient, and results showed that the ic similarity coefficients of 48 potato onion
germplasms ranged from to . Ten AFLP primers with rich polymorphism, stable amplification
and good repeatability were screened from sixty four AFLP primers. With these screened primers, 470
bands were identified, of which 288 bands were polymorphic. The ic similarity was estimated
with the Nei-Li coefficient, and results showed that the ic similarity coefficients of 48 potato
onion germplasms ranged from to . The clustering results of SSR and AFLP markers were
basically identical, and both methods showed that the cluster results of potato onion obviously
correlated with their geographic origins.
Key words: Allium cepa var. aggregatum; ic diversity; SSR; AFLP
0 Introduction
Potato onion (Allium cepa var. agrogatum Don.), which belongs to the Allium genera of Liliaceae
family, is an annual cultivation herbaceous plant. It is an important vegetable worldwide and
condiments because of it rich nutrients and high medicinal value, and widely cultivated in the northeast
area of China, such as Heilongjiang and Jilin Provinces. Because it has short growth period, not easy to
be affected by diseases or pests, and can e