文档介绍：
基于比较蛋白质组学筛选嗜酸氧化亚铁硫
杆菌硫氧化相关蛋白质#
彭安安1,夏金兰1,2,刘红昌1,朱薇1,聂珍媛1,2**
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(1. 中南大学资源加工与生物工程学院,长沙 410083;
2. 生物冶金教育部重点实验室,中南大学,长沙 410083)
摘要:在基于热水浴-Triton X-114 相分离特化空间蛋白质的基础上,基于比较蛋白质组学
方法和生物信息学分析,对在 Acidithiobacillus ferrooxidans 在元素硫和亚铁中培养的
细胞胞外、外膜和周质空间蛋白质进行研究,筛选与硫氧化密切相关关键蛋白。获得了 39
个与硫氧化相关的蛋白质,其中含有半胱氨酸残基的蛋白质约占 70%,并有部分蛋白质中半
胱氨酸丰度较高,含有一个到两个 CXXC 结构域。半胱氨酸残基上的巯基在硫氧化过程中具
有重要活性功能,位于细菌细胞包被中这些富含半胱氨酸的蛋白质的鉴出,表明这些蛋白质
在元素硫的活化/氧化过程中具有重要作用。
关键词:Acidithiobacillus ferrooxidans;比较蛋白质组学;硫氧化;生物信息学
中图分类号:TF18
Isolation of sulfur oxidation relevant proteins of
Acidithiobacillus ferrooxidans based parative
proteomics
PENG An-an1, XIA Jin-lan1,2, LIU Hong-chang1, ZHU Wei1, NIE Zhen-yuan1,2
(1. School of Minerals Processing and Bioengineering, Central South University, Changsha
410083;
2. Key Laboratory of Biometallurgy of the Ministry of Education of China, Central South
University, Changsha 410083, China)
Abstract: Based on water-bathing-Triton X-114 phase separation of the partment
-specific proteins, and parative proteomics and bioinformatic analysis, the extracullular,
out-membrane and perplasmic space proteins were studied to isolate the key proteins reltating to
the sulfur oxidation of Acidithiobcillus ferrooxidans 23270 that were cultured on substrates
elemental sulfur and ferrous iron, respectively. 39 sulfur oxidation related proteins were isolated
and identified. 70% of which contain cysteine residues, some have higher cysterine content and
one to two CXXC strucutral domans. Thio group of cysteine has important function in sulfur
oxidation, the identification of the cell envelpe proteins rich-in cysteine indicating these proteins
have key role in activation and oxidation of elemental sulfur.
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Key
words:
Acidithiobacillus ferrooxidans; comparative proteomics; sulfur oxidation;
bioinformatics
0 引言
嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)对元素硫的氧化是一个错综复杂
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的过程,一般认为发生在细胞周质中[1]。因此,细菌氧化代谢疏水性的元素硫首先必须对其
进行有效吸附或活化[2, 3],通过一定的