文档介绍：本论文的研究工作是在国家自然科学基金(30870502),北京j1,自然科学基金(5072037),国家自然科学基金杰出青年基金(30725009),卫生部公益性行业科研专项经费(20082007),长江学者和创新团队发展计划”项目(IRT0909),高等学校博士学科点专项科研基金(20070023021),中国医学科学院基础医学研究所院(所)长基金(2010PYZ20)的资助下完成的,在此表示诚挚的感谢!hisworkwassupposedbygrantsfrOmtheNationalNaturalScienceFoundation(30870502),theBeijingNaturalScienceFoundation(5072037),theNationalScienceFundforDistinguishedYoungScholar(30725009),theResearchGrantforPublicInterestfromMinistryofPublicHealth(20082007),Prog阳mforChangjiangScholarsandInnovativeResearchTeaminUniversity—PCSIRT(),SpecializedResearchFundfortheDoctoraIProgramofHigherEducation(20070023021),theDean’SGrantfromInsitituteofBasicMedicalSciences,ChineseAcademyofMedicalSciences/SchoolofBasicMedicine,PekingUnionMedicalCoIIege(2010PYZ20).Wewouldespeciallyliketoexpressourprofoundthanks!北京协和医学院博-上学位论文摘要目录摘要???????????????????????????????????3绪论??????????????????????????????????????????????.7第一章前言???????????????????????????????12PDZ结构域能够非传统地结合配体的中间序列???????????????????????????????????????????????????????????????????????????????????????????????????????18第二章中间序列随机八肽文库的构建????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????.?????????????????????????.39第三章PDZ结构域结合中间序列结合特性的研究????????????????.???????????????????????..???????????????????????????????????????????????????????????????????????????????????????(bait)的构建??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????