外周血干细胞骨髓腔内输注对移植物抗宿主病的影响.doc

文档介绍：外周血干细胞骨髓腔内输注对移植物抗宿主病的影响                      作者:毕延智,曾冬香,盛桂凤,张琰,陈宝安  【摘要】 本研究建立髓腔内外周造血干细胞输注(IBMPBSCT)动物移植模型并观察该方法对移植物抗宿主病(GVHD)的影响。选取雌性C57BL/6小鼠为移植受鼠,于第0天接受全身照射(TBI)4Gy后,输注经rhGCSF动员的雄性BABL/c小鼠外周血造血干细胞(1×107),2天后腹腔注射环磷酰***(CTX),观察各组小鼠GVHD发生情况。结果显示:髓腔内PBSCT组的受鼠GVHD发生率较尾静脉输注组明显减低(),移植后在受鼠体内观察到供鼠来源的Y染色体存在。结论:与尾静脉输注比较,髓腔内输注外周血造血干细胞不仅能减少GVHD的发生,而且更有利于干细胞植入。【关键词】 移植物抗宿主病  InfluenceofIntraboneMarrowInfusionofPeripheralHematopoieticStemCellson GraftVersusHostDisease  Abstract  Thisstudywasaimedtoexplorewhetherthegraftversushostdisease(GVHD)couldbealleviatedbyintrabonemarrow(IBM)(TBI)4Gyonday0,followedbyinjectionofperipheralhematopoieticstemcells(1×107)frommobilizedmaleBALB/cwith granulocytecolonystimulatingfactor(rhGCSF),andcyclophosphamide(200mg/kg) incidenceandseverityofGVHDweremorelowandalleviativeingroupIBMPBSCT  thanthat ingroupTVPBSCT().Ychromosomeofdoenormicecouldbedetected bonemarrowinfusionissuperiortoinjectionviathetailveinintheengraftmentofhematopoieticstemcellsintermsofstemcellhomingwhilethefrequency  andseverityofGVHDinallogeneicmicedecrease.  Keywords  hematopoieticstemcelltransplantation;intrabonemarrowinfusion;graftversushos