文档介绍：Biosensors based on surface plasmon-enhanced fluorescence
spectroscopy „Review…
͒
Jakub Dostáleka and Wolfgang Knoll
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
͑Received 2 May 2008; accepted 11 July 2008; published 16 January 2009͒
The implementation of surface plasmon-enhanced fluorescence spectroscopy ͑SPFS͒ to surface
plasmon resonance ͑SPR͒ biosensors enables increasing their sensitivity by several orders of
magnitude. In SPR-based biosensors, surface plasmons probe the binding of target molecules
contained in a liquid sample by their affinity partners attached to a metallic sensor surface. SPR
biosensors relying on the detection of refractive index changes allow for direct observation of the
binding of large and medium size molecules that produces sufficiently large refractive index
changes. In SPR biosensors exploiting SPFS, the capture of fluorophore-labeled molecules to the
sensor surface is observed by the detection of fluorescence light emitted from the surface. This
technique takes advantage of the enhanced intensity of ic field panied with the
resonant excitation of surface plasmons. The interaction with surface plasmons can greatly increase
the measured fluorescence signal through enhancing the excitation rate of fluorophores and by more
efficient collecting of fluorescence light. SPFS-based biosensors were shown to enable the analysis
of samples with extremely low analyte concentrations and the detection of small molecules. In this
review, we describe the fundamental principles, implementations, and current state of the art
applications of SPFS biosensors. This review focuses on SPFS-based biosensors employing the
excitation of surface plasmons on continuous metal-dielectric interfaces. © 2008 American Vacuum
Society. ͓DOI:
I. INTRODUCTION DNA hybridization at concentrations ,13 The refrac-
tive index changes were shown to be dramatically increased
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