文档介绍：Grandi
METHODS IN MOLECULAR BIOLOGY™• 375 METHODS IN MOLECULAR BIOLOGY™ 375
SERIES EDITOR: John M. Walker
In Vitro Transcription and Translation Protocols
In In and Vitro Transcription
Second Edition In Vitro
Edited by
Guido Grandi Transcription and
Biochemistry and Molecular Biology Unit, Novartis ines, Siena, Italy
A highly anticipated update of the previous edition, In Vitro Transcription and Trans-
lation Protocols, Second Edition, provides molecular biology laboratories with the most
powerful techniques for exploiting in vitro transcription and translation systems.
In Part One: Technologies, authors discuss the use of alternative energy systems for Translation Protocols
ATP regeneration and of continuous-exchange devices to obtain high levels of in vitro pro-
tein production, the efficient in vitro production of integral membrane proteins, a protein
category known to be extremely resistant to in vivo expression, and the high-throughput Protocols Translation
production of protein libraries using the innovative single-molecule PCR-linked in vitro
expression (SIMPLEX) technology. Other chapters also discuss the efficient, high-throughput Second Edition
protein production using the wheat germ in vitro expression system and exogenous protein
expression in Xenopus oocytes.
Part Two: Applications, authors present in vitro translation of amplified protease gene
from HIV patients to select proper antiprotease drug therapy and to follow the surge of drug Edited by
resistance, in vitro translation of genes from human patients to diagnose biologically relevant
gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries
of randomly produced lipase mutants, and the use of in vitro transcription and translation
to directly identify proteins in 2D-maps. Additional chapters investigate high-throughput Guido Grandi
in vitro expression of Bacillus anthracis antigens to identify ine candidates, the use of