文档介绍：
金银花鳞翅目害虫病原真菌的分离与鉴定#
马维思1, 2,徐常青1,张金良3,乔海莉1,郭昆1,徐荣1,陈君1,张大勇4**
(1. 中国医学科学院北京协和医学院药用植物研究所,北京 100193;
5
10
15
20
25
30
35
40
2. 云南省农业科学院药用植物研究所,昆明 650223;
3. 北京市植物保护站,北京 100029;
4. 加多宝集团种植资源部,北京 100176)
摘要:为了明确侵染金银花鳞翅目害虫的昆虫病原真菌的类型,利用无菌水冲洗受昆虫病原
真菌感染死亡的金银花鳞翅目害虫幼虫体表,获得病原真菌分生孢子液,将孢子液涂布在
PPDA 平板上培养、分离出昆虫病原真菌,依据形态特征和 ITS 序列特征,分离出的 12 株
真菌被鉴定为莱氏野村菌 Nomuraea rileyi。莱氏野村菌是鳞翅目昆虫较为常见的病原真菌,
能够在某些害虫间形成流行病,有较大的开发价值,可作为开发针对药材鳞翅目害虫的真菌
杀虫剂的候选材料。
关键词:金银花;鳞翅目幼虫;莱氏野村菌
中图分类号:
Isolation and Identification of entomopathogenic fungi from
Lonicera japonica Thunb pest
MA Weisi1, 2, XU Changqing1, ZHANG Jinliang3, QIAO Haili1, GUO Kun1, XU
Rong1, CHEN Jun1, ZHANG Dayong4
(1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking
Union Medical College;
2. Institute of Medicinal Plant,Yunnan Province Agriculture Academy of Science;
3. Beijing Plant Protection;
4. JDB Group)
Abstract: In this study,twelve samples of lepidoptera larvae infected by entomopathogenic fungi
were collected, twelve entomopathogenic fungi strains were isolated by culturing spores that
clustered round the dead larvae, on the basis of morphological characteristic and rDNA ITS
sequence, all strains of entomopathogenic fungi were identified as Nomuraea rileyi, and they will
be useful research materials for further study to control insects of Lonicera japonica T