文档介绍：
玉米耐旱相关基因 dbf1 的序列变异分析#
杨泽峰,张恩盈,蒋莹,徐扬,潘亮,陈庆,徐辰武**
(扬州大学农学院教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室,
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江苏扬州,225009)
摘要:玉米 dbf1 基因在干旱胁迫下的编码产物与 DIP1 蛋白相结合,调控 ABA 响应基因 rab17
的表达,从而提高耐旱性。为研究玉米 dbf1 基因的序列多态性,以 B73 的 dbf1 基因组序列
为模板,对 104 个玉米自交系的 dbf1 基因进行了测序。多态性分析表明,在全长为 2118bp
的序列中共发现了 48 个 SNP 和 12 个 InDel。尽管大部分变异位点集中在非编码区,但编码
区的 1 个 InDel 和 3 个非同义突变位点的变异可以产生氨基酸序列改变。玉米 dbf1 基因的
全长序列和编码区序列的变异位点可以分别将该基因划分成 33 和 11 种单倍型,并且编码 7
种蛋白质。在供试材料中,dbf1 基因至少经历了 9 次重组,中性检测表明该基因的进化没
有偏离中性选择。
关键词:作物遗传育种;dbf1 基因;单核苷酸多态性(SNP);InDel;连锁不平衡
中图分类号:请查阅《中国图书馆分类法》
Analysis of nucleotide diversity of drought tolerance related
maize gene dbf1
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Yang Zefeng, Zhang Enying, Jiang Ying, Xu Yang, Pan Liang, Chen Qing, Xu
Chenwu
(Jiangsu Key Laboratory of Crop ics and Physiology/Key Laboratory of the Ministry of
Education for Plant Functional Genomics, Yangzhou University, Yangzhou, Jiangsu, 225009)
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Abstract: The protein product encoded by maize dbf1 gene bine with DIP1 protein to
regulate ABA responsive gene rab17 expression under drought stress, resulting in improvement of
drought tolerance. In order to illustrate the sequence polymorphism of maize gene dbf1, we
sequenced the dbf1 gene in 104 maize inbred lines, using the genomic sequence of B73 as the
reference. A total of 48 SNPs and 12 InDels were identified among 2118 bp nucleotide sequences
at this locus. Although most of these polymorphic sites were found to be located in non-coding
regions, 1 InDel and 3 nonsynonymous mutations in coding regions may change the amino acid
sequences. The polymorphic sites in the genomic sequences and the coding sequences of maize
dbf1 classified all the tested inbreds into 33 and 11 haplotypes, respectively. In addition, we
noticed that 7 deferring DBF1 proteins were encoded by these inbred lines. Analysis of the
polymorphism sites revealed that at least 9 bination events have occurred in dbf1 locus.
Furthermore, the evolution of maize dbf1 gene in the tested lines