文档介绍：
云南虫生真菌粉棒束孢遗传分化研究#
代永东,虞泓,陈自宏,杨俊媛**
(云南大学中草药生物资源研究所云百草实验室,昆明 650091)
5
10
15
20
摘要:对云南粉棒束孢 8 个当地居群和蝙蝠蛾拟青霉 2 个当地居群进行 ITS 测序和 RAPD
扩增分析,结合 GenBank 中相关序列,对粉棒束孢开展遗传多样性、居群遗传结构及其种
内分化研究。共获得大范围内地理距离远的 6 个居群共 97 条粉棒束孢 ITS 序列,分 33 种单
倍型,单倍型多样性 Hd= 和总核苷酸多样性 Pi=,显示在物种水平上粉棒束孢
遗传多样性较低。云南地区粉棒束孢共 37 条序列,分 14 种单倍型(10 种为云南地区特有),
具有较高单倍型多样性和核苷酸多样性(Hd=,Pi=);单倍型聚类和网状分支
分析表明云南地区粉棒束孢遗传多样性程度高且单倍型类型丰富,暗示云南地区为粉棒束孢
多样性分布中心之一。云南 8 个当地居群 ITS 序列在居群间遗传分化系数 Fst=%,RAPD
电泳图谱图谱分析在居群遗传分化系数 Gst=,基因流 Nm=,说明云南当地居群
粉棒束孢分化剧烈。居群平均遗传距离与地理距离相关性研究表明粉棒束孢遗传距离与地理
距离无明显相关。中性检验和失配分析表明粉棒束孢经历过近期居群扩张。结合单倍型聚类
和网状分支分析结果表明 Hap 19 为扩张建群单倍型,但原始祖先单倍型(Hap 1)依然是居
群中最优势单倍型(频率为 %),表明粉棒束孢并不存在明显的因地理原因在造成的
生殖隔离;现有粉棒束孢遗传格局是原始单倍型及其衍生物与建立者单倍型衍生类群共同存
在并相互作用的结果。此外,8 条蝙蝠蛾拟青霉 ITS 序列分 4 种单倍型,其中 3 种与粉棒束
孢共享,另一个特有单倍型 Hap 34 为粉棒束孢优势单倍型 Hap 1 基本衍生型;RAPD 结果
也显示蝙蝠蛾拟青霉不能与粉棒束孢分开,这些结果支持将蝙蝠蛾拟青霉作为粉棒束孢的同
物异名处理。
关键词:居群遗传学;ITS;RAPD;单倍型;居群扩张;相关性
中图分类号:请查阅《中国图书馆分类法》
25
ic differentiation of an entomogenous fungus Isaria
farinosa in Yunnan, China
DAI Yongdong, YU Hong, CHEN Zihong, YANG Junyuan
(Yunnan Herbal Laboratory, Institute of Herbal Biotic Resources, Yunnan University, Kunming
30
35
40
650091)
Abstract: In order to detect the ic diversity, population ic structure and intraspecific
differentiation of Isaria farinosa, the ITS sequencing and RAPD amplification were conducted in 8
local populations of Isaria farinosa and 2 local populations of Paecilomyces hepiali in Yunnan.
Combined with the ITS of Isaria farinosa in GenBank, a total of 97 ITS sequences were obtained
in the 6 populations from widely separated geographical locations. Among them, 33 haplotypes
were discovered in the present paper. The haplotype (gene) diversity was Hd= and
nucleotide diversity was Pi= , showing lower degree of ic diversity at the species
level of Isaria farinosa. A total of 14 haplotypes were found in the local populations of Yunnan,
and 10 of these were not foun