核定位信号肽提高核糖体区打靶载体转染效率的研究.pdf

文档介绍：生物化学与生物物理进展
ProgressinBiochemistryandBiophysics
2009,36(10):1283~1290

TheTransfectionEfficiencyImprovementof
hrDNATargetingVectorsWithNLSPeptide*
SHIYan**,LIUXiong-Hao**,LIANGDe-Sheng***,FENGMai,WULing-Qian,YANGJun-Lin,
LIZhuo,ZHAOKai,PANQian,LONGZhi-Gao,WULing-Qian,XIAJia-Hui
(ics,CentralSouthUniversity,Changsha 410078,China)
Abstract Humanribosomalgene(hrDNA)targetingvectors(10~ 14kb)constructedbythegrouparenovelnon-viralvectorswhich
couldspecificallyintegrateintotheribosomallociandcharacterizedbytheirsecurityandstableexpressionoftherapeuticgenes.
However,(NLS)could
facilitatethenuclearentryofnon-viralvectorsandimprovethetransfectionefficiency,theexpressionoftherapeuticgenevaried
-
[4-(psoralen-8-yloxy)]butyrate(SPB)withSimianVirus40NLS(SV40NLS)peptidethroughelectrostaticinteractionefficiently,
whichcouldprotecttheplasmidDNA(pDNA)(PEI),whichisaneconomicaland
lowtoxicpolymerandwildlyused invivo herapy,wasemployedtotransfecttheprimaryhumandermalfibroblasts(HDF).When
conjugatedwithNLSpeptide,
GFPfluorescenceanalysisbyflowcytometryshowedthatthetransfectionefficie