文档介绍：Effects of(-)epigallocatechin gallate on expressions of and hMLH1 hMSH2【ABSTRACT】OBJECTIVE: To investigate the effect of (-) epigallocatechin gallate(EGCG) on mRNA expressions of hMLH1 and hMSH2 in human lymphoblast cell lines. METHODS: Total RNA was isolated from GM12593 and GM13705 cells treated with EGCG(0,5,10,20 μmol/L) for 6 days. Real-time fluorecence quantification PCR(FQ-PCR) was conducted to measure the mRNA expression levels of hMLH1 and hMSH2. RESULTS: The mRNA expressions of hMLH1 and hMSH2 significantly increased at 20 μmol/L EGCG in GM12593 cells,and were significantly higher than equal concentrations in GM13705 cells. The effect of EGCG on expressions of target genes were insignificant in GM13705 cells. CONCLUSION: EGCG had the potential to up-regulate the expressions of hMLH1 and hMSH2, contributed to the promotion of mismatch repair and maintaining genomic stability in normal human lymphoblast cell line GM12593. 【KEY WORDS】 (-)epigallocatechin gallate;human lymphoblast cell line;mismatch repair gene;gene expression【摘要】 目的: 探讨表没食子儿茶素没食子酸酯[(-)epigallocatechin gallate ,EGCG]对人成淋巴细胞株错配修复基因hMLH1和hMSH2 mRNA表达水平的影响。方法:将正常人成淋巴细胞株GM12593和乳腺癌患者成淋巴细胞株GM13705分别置于含有0、5、10、20 μmol/L EGCG的RPMI-1640中进