文档介绍：13
论著
July
The effect and mechanism of
人参多糖与他莫
昔芬联合对人乳 ginseng haride on
腺癌MCF-7细胞 tamoxifen -induced apoptosis
凋亡的影响及其 in human breast cancer
机制研究 MCF-7 cells
刘文娟1 /高●鹏2 /王大海2
( 1. 哈尔滨医科大学附属第二医院感染内科,
1 2 2
黑龙江●哈尔滨● 150081;2. 哈尔滨市儿童医院 LIU Wen-juan ,GAO Peng ,WANG Da-hai
心脏外科,黑龙江●哈尔滨● 150010 ) (1. Department of Infectious Disease, The Second Affiliated Clinical
Hospital of Harbin Medical University, Harbin 150081; 2. Department
of Cardiac Surgery, Harbin Children’s Hospital, Harbin 150010,
Heilongjiang, China)
【摘要】目的: 探讨人参多糖(ginseng haride,GPS)与他莫昔芬(tamoxifen,TAM)联合对人乳腺癌MCF-7细胞凋亡的影
响及其机制。方法:以不同浓度(0、20、40、80和160 μg/mL)GPS作用于人乳腺癌MCF-7细胞株48 h,以MTT法检测细胞增殖抑制
情况,以GPS的最小有作用剂量与不同浓度(0、、1、2和4 μg/mL)TAM联合处理细胞48 h,以金氏公式筛选出协同抑制作用最
明显的联合剂量,以该联合剂量处理MCF-7细胞48 h后,用DAPI染色法、流式细胞术检测细胞凋亡情况;用Giemsa染色检测细胞
有丝分裂指数;用Western blot检测细胞中Fas、Caspase-9 以及Parp的表达情况。结果:MTT法检测提示GPS在48 h对MCF-7细胞的
最小有作用剂量为40 μg/mL,金氏公式计算结果提示40 μg/mL GPS+1 μg/mL TAM联合用药协同抑制细胞增殖作用最强
(q=);与GPS或TAM单独作用相比,DAPI染色发现联合用药组镜下可见大量凋亡小体;流式细胞仪检测发现,联合用药能够协
同增加细胞凋亡率( q=,P <);Giemsa染色结果显示联合用药能够协同抑制细胞有丝分裂(均P<);Western blot检测发
现,联合用药能增加细胞中Fas表达,促进Caspase-9以及Parp的活化。结论:GPS与TAM联合能够协同通过Fas信号通路促进人乳
腺癌MCF-7细胞发生凋亡。
【关键词】人参多糖;他莫昔芬;凋亡;MCF-7细胞
中图分类号: 文献标识码: A 文章编号: 1004-616X(2013)04-0280-05 doi:.1004-
【 ABSTRACT】 OBJECTIVE: To study the effect and mechanism of ginseng haride (GPS) on tamoxifen
(TAM)-induced apoptosis in human breast cancer cells. METHODS: Human breast cancer cells MCF-7 were treated
with different doses of GPS for 48 h. MTT assay was used to evaluate the minimal effective GPS dose. Then cells were
treated with GPS at minimal effective bined with different doses of TAM. Bürgi formula was used to assess the
synergy effect between GPS and TAM. Cellular mitosis was detected by Giemsa staining assay. Apoptosis was studied by
DAPI staining analysis and flow cytometer. The expression of Fas,Caspase-9 and Parp was m