文档介绍：摘要零论文浆雳求裁煮法帮蠢酵浸提法瓣决褥予畜效成分进行了掇澈,绪莱袭弱乙静提取漩优于水浸提法。经优化质碍到己醇浸掇法的掇取条件为:70%浓度的乙醇,1S倍髂虢懿窳入譬,浸攥溢爱为50℃,浸撵辩瀚为藤。并爨褥掰乙醇浸提麓最透罐取次数为2次,决明子在矗醇浸樾优化条件下最多可得到的蒽醌含量为O。50%o。零论文又辩凌骥予霄效簸分戆分耩方法滋行了磷究,稳定TLC徐灞耱袋羚裁为簸仿:即醇:水一5:3;1:HPLC色谱条件为:ODS2反相柱(Srma,×250mm);检测渡长:280nm;犍澄:25℃;流速,lml/min;7-,筵一窳裙发洗巍(0min,乙蘑1%;5min,乙精40%:15rain,乙腈55%;25rain,厄腈75%i30min,乙腈90%;35rain,乙腈100弼)。裙杌溶粥攀取法分离决瞒予耱提钫得到5个不阍极性组分,得率分剐为:裕油醚艨,9,94%;乙醚鼷,5。%%;己酸己翡层,3.?霹%;歪T酪藤,13+85豫拳瑟,%;AB*8太孔树耱吸戳臻析法分离决骥予租掇物,得潮5个不犀维分,得率分裂为:%、丛。14%、2,69%、186%、7。63%;毽羧毽瑟毒蓐分裹决甥予糕糖貔餐溺了7个不霹缀分,%、5,75%、4+97%、7,59%、%、%、%。经TLC检测醚黢撞髅据分囊效果饶予其它嚣秘方法。采用转入雌激素麓嚣酵母测定决明予分离组分豹体终麟激素活性,结果执聩子辩乙酸乙聚罄垃、遵丁酵鄹位移穗胶靛分舞蕊第4缀势体努鹾激素溪性较羰。关键谢;狭嬲予,有效成分,撵凝,分离,体外雌激素活健AbstractAbstractInthispaper,(70%)at50℃for4hwasbetter,%,∞Chloroform—methanol-water(5:3:1,v/v).弛eoptimumconditionsofHPLC~VCl”e;ODS2eolumn(5pm,)(250mm);testwaveleng出,280nm;cohmmtemperature,25.{2;flow,lml/min.-Waterwas:0minute,1%;5minute,40%;15minute,55%;25minute,75%;30minute,900,;35minute,l∞%.Fivefractionswereseparatedbyo糟amesolventextraction,andt