文档介绍：河北大学
硕士学位论文
马鞭草对荷瘤小鼠抑瘤作用及免疫功能影响
姓名:李小龙
申请学位级别:硕士
专业:药理学
指导教师:孟明
2010-12
摘要
摘要
目的
通过研究马鞭草的水提取物和醇提取物对 H22 肝癌小鼠的体重、肿瘤生长及脾指数
等影响,并进一步研究马鞭草水提取物及其与顺铂联合应用对肝癌小鼠的抑瘤效应和免
疫器官、体液免疫、细胞免疫等免疫指标的影响,探讨马鞭草对肝癌小鼠抑瘤及免疫功
能的影响。
方法
、化疗对照组、马鞭草醇提取物组及水提
取物组;末次给药后 24 小时检测下列指标:体重变化、抑瘤率及脾指数的测定。
,实验动物肿瘤接种同前,随机分为肿瘤对照组、化疗对照组、水提
物+化疗组及单纯水提取物组,末次给药后 24 小时检测下列指标:体重变化、抑瘤率、脾指
数、迟发性变态反应(DTH)、血清溶血素水平、血清 IL-2 水平、脾细胞增殖反应的测定。
结果
,抑瘤率分别为 %和 %;马
鞭草醇提取物和水提取物组脾指数、体重增长较对照组和顺铂组降低,但无显著性差异。
,抑瘤率为 %。
同时体重增长明显低于对照组、单纯顺铂组及单纯水提取物组。
、DTH、血清溶血素水平、血清IL-2
水平及脾细胞增殖明显低于对照组和单纯顺铂组。单纯马鞭草的水提取物组荷瘤小鼠的
脾指数、DTH、血清溶血素水平、血清IL-2 水平及脾细胞增殖与对照组相比无显著性差异。
结论
马鞭草的醇提取物和水提取物均可抑制荷瘤动物体内的肿瘤生长;马鞭草水提取物
与顺铂联合应用具有增强抑瘤作用;单纯马鞭草的水提取物对肝癌荷瘤小鼠的免疫功能
没有损伤作用,但与顺铂联合应用对肝癌荷瘤小鼠的脾指数、DTH、血清 HA 水平、IL-2
活性及脾细胞增殖具有降低作用。

关键词:马鞭草提取物抑瘤作用免疫功能小鼠
I
Abstract
Abstract
Objects
To study the effect of european vebena on body weight tumor growth and spleen index’s
functions of tumor-bearing H22 mice. Further,to study the effect of water extraction from
european vebena and plus chemotherapy on the tumor inhibitory and an,humoral
immunity,cell immunity etc.
Methods
One part the mice with H22 were divided into control and cisplatin groups,Other were
given extraction of european vebena by weight,tumor weight,spleen weight were
part according to above-mentioned,The mice with H22 were divided into
control and cisplatin groups, Other were given water extraction of european vebena plus
weight, tumor weight,spleen weight,DHT,HA,IL-2 and spleen generation
were measured.
Results
The results showed that both the water-extraction and the alcohol-extraction of european
vebena inhibited the growth of the tumor. The rats of tumor-inhibition were % and %
respectively in the experimental groups and the controls. The growth of weight and sple