文档介绍：M ETHODS IN MOLECULAR BIOLOGY™
Series Editor
John M. Walker
School of Life Sciences
University of Hertfordshire
Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes:
ies/7651
Gene Synthesis
Methods and Protocols
Edited by
Jean oud
Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA, USA
Editor
Jean oud, .
Virginia Bioinformatics Institute
Virginia Tech
Blacksburg, VA, USA
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ISSN 1064-3745 e-ISSN 1940-6029
ISBN 978-1-61779-563-3 e-ISBN 978-1-61779-564-0
DOI -1-61779-564-0
Springer New York Dordrecht Heidelberg London
Library of Congress Control Number: 2012930137
© Springer Science+Business Media, LLC 2012
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Preface
The de novo fabrication of custom DNA molecules is a transformative technology that
signifi cantly affects the biotechnology industry. Basic ic engineering techniques for
manipulating DNA in vitro opened an incredible fi eld of opportunity in the life sciences.
However, ic engineering has now moved beyond the introduction of single genes into
cells to multigene cassettes, and is rapidly progressing toward whole genome engineering.
In th