文档介绍：Protein Synthesis Initiation Secondary article
in Prokaryotes Article Contents
. Introduction
Marianne Grunberg-Manago, Institute of Physico-chemical Biology, Paris, France . Messenger RNA Selection – Shine and Dalgarno
Interaction
.
The term ‘translational initiation’ refers to the sequence of events leading up to the Structure and Function of Initiation Factors (IFs)
.
formation of the first peptide bond. It is usually the rate-limiting step of translation and its Pathway of plex Formation
.
efficiency can vary by several orders of magnitude. It is therefore a major determinant of the Role of IF2-dependent GTPase
.
overall expression level for a gene and is a target for a variety of translational control Ribosomal Binding Sites in the plex
.
mechanisms. Acknowledgements
Introduction
understanding of translational initiation (Shine and
The pathway for the formation of the translation initiation Dalgarno, 1974). Numerous experiments support the idea
complex involves numerous individual reactions, the large that this sequence is involved in initiation site selection: (1)
majority of which are ‘noncovalent’ in nature. The only the 3’ terminal sequence of 16S rRNA is highly conserved
‘covalent’ step is the hydrolysis of guanosine triphosphate and accessible to mRNA; (2) ple-
mentary to this region of 16S rRNA inhibit translation; (3)
(GTP) to guanosi