文档介绍：浙江大学博士学位论文致谢行同步分析,总分析时间约8 min。关键词:微流控;非均相免疫分析;毛细管;缺口管阵列;自动化;人IgG;蠕动泵 IV 浙江大学博士学位论文 Abstract Abstract Immunoassay is awell-established analysis technique with high specificity and sensitivity based on specific interaction between antigen andantibody,and has been widely used inclinicaldiagnosis,biochemical analysis,environment monitoring and food safety immunoassay,especially enzyme—linked immunosorbent assay(ELISA),is the most important andwidely—used quantitative method in the field ofclinical ,in most of the traditional immunoassays,the operations are usually carried outmanually,which are tedious and time-consuming,and the consumption forsample and reagent isamong several hundreds ofmicroliters. Microfluidics isthe science andtechnology thatmanipulate small(nL—pLscale) amount of fluids in pm—scale channels and microfluidic systems。Microfluidic analytical systems have the advantages of fastanalysis,low sample/reagent consumption and potential for integration and of microfluidic technique wim immunoassay provides significant improvements in analysis speed and throughput,sample/reagent consumption,and integration and automation ofthesystem. Inchapter 1,theprogress inmicrofluidic immunoassay systems,especially in automated heterogeneous immunoassay,is reviewed. Inchapter 2,an automated microfluidic immunoassay system was developed based on capillary and slotted—vialarrays,in which fully automated operation for multi-step immunoassay was system was applied intheimmunoassay ofhuman assay for ninehuman IgG samples was achieved within 0min. The limitofdetection forIgG was precision ofthesystem was % (RSD,n29).The sample andreagentconsumption was “L for each cycle. Inchapter 3,a microfluidic immunoassay system Was developed based on slotted—vialarray,microperistaltic pump andPDMS peristalticpump with a micro—gear was usedforliquiddriving insixmicrochannels inPDMS chi