光合细菌菌剂在提高板蓝根品质中的应用研究.doc

文档介绍：光合细菌菌剂在提高板蓝根品质中的应用研究
[摘要]目的:考察在板蓝根种植中应用光合细菌菌剂后板蓝根和大青叶药材品质的提高。方法:采用高效液相色谱法,以甲醇-水(75:25)为流动相,检测波长为 289 nm,分析大青叶中靛玉红的含量;按药典要求,用 45%乙醇做溶剂测定板蓝根中醇溶性浸出物的含量。结果:使用光合细菌菌剂后,大青叶中靛玉红含量由 mg/g 增加为 mg/g,%;%%,%。结论:光合细菌菌剂可以提高板蓝根和大青叶的质量。
[关键词]光合细菌;高效液相色谱法;板蓝根;大青叶;靛玉红
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Application of Photosynthetic Bacteria in improving the quality of Radix Isatidis
[Abstract] Objective: To improve the quality of Radix Isatidis and Folium Isatidis by applying Photosynthetic Bacteria.
Methods: HPLC method was used for analyzing the content of Indirubin in Folium Isatidis with methanol-water (75:25) as
the mobile phase and detecting wavelength at 289 nm. According to Pharmacopoeia, 45% ethanol was used as solvent to
detect the content of ethanol-soluble extractive in Radix Isatidis. Results: After using Photosynthetic Bacteria, the content
of indirubin in Folium Isatidis increased from mg/g to mg/g, increased %. The content of ethanol-soluble
extractive of Radix Isatidi increased from % to % , increased % . Conclusion: The quality of Radix Isatidis
and Folium Isatidis can be improved by applying Photosynthetic Bacteria.
[Key words] Photosynthetic Bacteria; HPLC; Radix Isatidis; Folium Isatidis; Indirubin
光合细菌菌剂是一种生物肥料
[1-3]
,它是以自配培养基为
原料、活性光合细菌为菌种,经现代生物工程技术研制而成
的一种复合性生物肥料。具有生物固氮,分解硫化物、农药、
化肥等多种有毒有害物质的能力
[4]
,可有效降解土壤中的农
药残