文档介绍：FOUNDATIONS
IN CANCER RESEARCH
The Turns of Life and Science
Jan Svoboda
Institute of Molecular ics, Academy of Sciences of the Czech Republic,
Vı´denˇska´ 1083, 142 20 Prague 4, Czech Republic
I. To Write or Not to Write?
II. Boys and Resurrection of Czechoslovakia
III. University
IV. Virogenic Cells and Provirus Integration
V. Virus Rescue
VI. Prague Spring 1968
VII. Dark Years
VIII. Partial Thawing and Molecular Biology
IX. Silencing the Provirus and the Red Power
X. Epilogue
References
Comme Sodome et Gomorre puissez tomber en soulfre en feu & abysme, en cas que
vous ne croyez fermement tout ce que je vous pteray en ceste presente chronicque.
Rabelais, F.: Gargantua and Pantagruel
Like those of Sodom and Gomorrah, may you fall into sulphur, fire and bottomless pits,
in case you do not firmly believe all that I shall relate unto you in this present Chronicle.
Rabelais, F.: Gargantua and Pantagruel (translated by Sir Thomas Urquhart of
Cromarty and Peter Antony Morfeus)
This chapter provides a personal insight into the scientific and social atmosphere in
former Czechoslovakia. It covers the period of the rise of Hasˇek’s immunologic school and
application of immunologic tolerance to Rous a virus (RSV) heterotransmission.
These approaches permitted establishment of a new model of mammalian cells transformed
by RSV (virogenic XC cells), where the noninfectious viral genome was kept indefinitely as
new ic information (provirus). RSV was rescued from nonpermissive mammalian cells
by fusion (complementation) with permissive chicken fibroblasts; this opened the way to
understanding virus nonpermissiveness. Mammalian cells transformed by the reverse
transcript of v‐src mRNA were characterized, and the resulting provirus was shown to be
highly oncogenic for chickens and to carry tumor‐specific transplantation antigen. Other
areas covering ic reversion of RSV‐transformed cells and long‐term persistence of
chic