文档介绍:--(primerlocationsareindicatedbyarrows,seeMaterialsandMethodsforsequences).-base-pairfragmentwasamplifiedfromtheoriginal,GFP-containingconstruct,whileCre-mediateddeletionledtotheappearanceofa502-base-:MWmarkers;Lanes2and3:controlplasmidsasshownintherightsideofthefigure;Lanes4and5:TransgenicmicegeneratedusingtheGFPexpressinglentiviralconstruct;Lanes6to10:G3miceobtainedaftercrossingaG2transgenicmousewithaPGK--7-2-1-3and-4lackexpressionofthetransgene,whileG3A57-7-2-1-1,-2and-5expresstheAnkrd57geneunderthecontroloftheCAGpromoter.