文档介绍：上海闪晶生物代理
न喉GTI喊 Certified ooL
٤ഓ߅ࠚ
㗡⺛〚䅓䄨㡘⹌㯟ᰃ⬅ᮙ㕢ढռ䪅ᰧ吓म˄Dr. ˅Ѣ 2001 ᑈ߯
ࡲⱘ催ᡔᴃӕϮˈ݀ৌᘏ䚼ԡѢ㕢⸙䈋ˈЏ㽕ҷ⧚ⱘᰃ ABIǃAXONǃBECKMAN ঞ
݊Ҫⶹৡॖଚⱘ⾡Ҿ఼䆒ˈབ PCR Ҿˈ㤻ܝᅮ䞣 PCR Ҿˈ⌟ᑣҾˈড়៤Ҿˈ㢃⠛
ᠿᦣҾǃ䍙䗳⾏ᖗᴎㄝˈϮࡵ䘡ঞ㕢ঞ㽓ㄝᆊˈ䖥ᑈᴹϢЁᕜ催ㄝ䰶᷵ǃ⾥
ⷨ䰶᠔Ϯࡵᴹᕔकߚ乥㐕DŽ᠔ଂҾ఼ӋḐᵕ݊ӬᚴDŽ∖ӋḐ᳔ԢˈᗻӋ↨᳔催DŽ
៥Ӏ݀ৌҷ⧚ⱘҾ఼Ўሩ㾜⫼ḋકҾ఼ˈ᠔᳝Ҿ఼ଂߎࠡ䛑㒣䖛 ABI ϧϮᎹ
ᏜϹḐⱘ䇗䆩ǃẔ⌟ǃ᷵ޚˈ䋼䞣ϢߎॖᯊϹḐϔ㟈DŽ៥Ӏ݀ৌᢹ᳝ᅠⱘଂৢ᳡ࡵԧ
ˈ䌍ֱׂˈϔᑈৢֱׂাᬊӬᚴⱘ䜡ӊ៤ᴀ䌍ˈݙᕜ催ㄝ䰶᷵ܡҾ఼ଂߎϔᑈݙˈ㋏
⾥ⷨ䰶᠔䛑ᰃ៥Ӏⱘ⫼᠋ˈ៥݀ৌ䰚Ꮖᓎゟ䍋њ㡃དⱘໄ䁝DŽ
䖯ⱘᅲ偠Ҿ఼ᓩ䖯ࠄܜߚњ㾷ݙ⾥ⷨথሩ䍟ˈᇚܙ៥݀ৌⱘӬѢ
㗗ᆳ⫮㟇䆩⫼ݡфⱘ䫔ଂԧ㋏ˈᅠ਼ࠄⱘଂৢᡔܜݙˈҹᵕԢⱘӋḐˈᮍ֓Ⳉ㾖ǃ
ᴃ᳡ࡵԧ㋏ˈЎݙ⫳⠽⾥ᡔǃए⭫Ꮉ㗙ᦤկϔ⌕ⱘѻક᳡ࡵDŽ
䰸њⳂᔩϞⱘϔѯЏ㽕Ҿ఼П,݊Ҫⱘϔѯ⾥ⷨࡲ݀Ҿ఼ˈབᵰᙼ䳔㽕ⱘ䆱ˈ៥
Ӏгৃ㛑ҹᕜӬᚴⱘӋḐ䖯㸠ҷ⧚䫔ଂˈᏠᳯᙼ㛑䎳៥Ӏ㘨㋏DŽ
˝ࡴ۞ᐎ໕ݨଖᆼᏪ˨༯ᐎܹݨଖᆼᄵሰ
˝ሞᎅᅓݨቭࢯ˨ၞໂݡݨ༵७࡞Ⴣ
৻ޔPCR Ҿ
ǃ$%, ൟ 3&5 Ҿggggggggggggggggggggggggg
2ǃMJ PTC-200 PCR Ҿggggggggggggggggggggggggg
ੑޔᅲᯊᅮ䞣 PCR Ҿ
1ǃ$%,㤻ܝᅮ䞣 3&5ggggggggggggggggggggg
2ǃABI 7700 㤻ܝᅮ䞣 PCRggggggggggggggggggggg
3ǃBio-Rad I-cycler 㤻ܝᅮ䞣 PCRgggggggggggggggg
ਃޔ⌟ᑣҾ
1ǃ$%,35,60嘐 ൟܼ㞾ࡼ↯㒚ㅵ⌟ᑣҾgggggggg
2ǃABI PRISM® 3700 ൟ↯㒚ㅵ⌟ᑣҾgggggggggggg
3ǃAmersham MEGABACE 1000 ⌟ᑣҾggggggggggg
྾ޔḌ䝌ড়៤Ҿ
1ǃ$%, Ḍ䝌ড়៤Ҿgggggggggggggggggggggggg
2ǃABI 394 Ḍ䝌ড়៤Ҿggggggggggggggggggggggggg
ޔ⫳⠽㢃⠛ᠿᦣҾ
1ǃ$[RQ$ ⫳⠽㢃⠛ᠿᦣҾgggggggggggggggggg
2ǃ$[RQ% ⫳⠽㢃⠛ᠿᦣҾgggggggggggggggggg
ޔ䍙䗳Ԣ⏽⾏ᖗᴎ
Beckman 䍙䗳Ԣ⏽⾏ᖗᴎ L8-80Mgggggggggggggggg
Beckman 䍙䗳Ԣ⏽⾏ᖗᴎ XL 90ggggggggggggggggg
৾ޔܝᄺᠿᦣ㋏㒳
㒳gggggggggggggggggggggg㋏ڣ៤ܝ▔Odyssey 㑶
㒳ggggggggggggggggggg㋏ڣAmersham storm 860 ᕅ
㒳gggggggggggggggg㋏ڣAmersham Typhoon 8600 ᕅ
PCR ሑ
$%, ൟ 3&5 Ҿ
˄ABI 9700 Thermal Cyclers˅
9700 ൟ PCR ҾᰃЎ PCR Ḍ䝌ᠽ
ᡔᴃ᠔䆒䅵ⱘ㞾ࡼ࣪Ҿ఼ˈᅗ᳝ PE
݀ৌ 9600 ൟ PCR Ҿⱘᗻ㛑 2400 ൟ
PCR ⱘ᪡⬠䴶ˈ⫼᠋⬠䴶ࣙᣀ᭄ܼᄫ
ᓣ䬂Ⲭˈ䕃㾺ᩌᣝ䬂⎆㸼ᓣሣ
ᐩᰒ⼎ሣDŽ↣Ͼ PCR ᮍ⊩䖤㸠݊ᯊ䯈
ᄬټ⏽ᑺᑣӮᅲᯊࡼᗕᰒ⼎ˈ᳔ৃ
100 Ͼᅠᭈⱘ PCR ᮍ⊩DŽ9700PCR Ҿ䜡㕂
ϔϾৃᤶⱘ 96 ᄨ ㅵḋકᑻˈ
ᇚᴹгৃ䜡㕂݊ᅗḋકᑻᔶᓣˈࣙᣀՓ⫼ ḋકㅵⱘḋકᑻՓ⫼ 384 ᄨⱘḋક
ᑻDŽ
ᗻ㛑⡍⚍˖
㪱㡆ሣᐩ⎆ᰒ⼎ሣˈऩሣᰒ⼎᳔䖒 280 ᄫ㡖ˈ㸼ᓣ PCR ᠽᮍ⊩ˈ᭄䕧ܹ
㸼ϔ㠀ˈࡴ䗳ㅔ֓Ⳉ㾖ˈᑣ䖤㸠ৃ䮾⚕ᰒ⼎ᔧ฿ڣ᮴䳔ᥠᦵӏԩ⡍⅞ੑҸˈPCR ব៤
ࠡ⢊ᗕDŽ
ḋકᑻᴀ䜡㕂ৃᤶൟ 96 ᄨᑻˈϔϾЏᴎৃ䜡⾡ϡৠ㉏ൟⱘḋકᑻˈ㗠Ϩ
䇗ᤶⳌᔧᮍ֓ㅔ֓ˈҹ⒵䎇ϡৠ PCR ডᑨ