文档介绍：小鼠脾脏细胞培养PRIMARYCULTUREOFMOUSESPLEENCELLS&IDENTIFICATIONOFLIVEORDEADCELLSBYDIFFERENTIALSTAININGQianqianChen(1**********)Teammembers:QingqingYu,QingZhangApril7th2012AbstractCellsfrommuti-anismcanbeisolatedandculturedaliveinvitro,,ourteamculturedthemousespleencellsinvitroandfondthatthoughthecellsweremicrobio1ogicalcontaininated,thecelIssomehowsurvived,,,,thispreessiscalledcellsenescence,eimmortal,,somedyescanpermeatethemembianeofthelivecells(%EosinY),butsomecannot(1%(w/v)Trypanblue).%EosinY,butonlythedeadcellscanbecolouredby1%(w/v)%(w/v)%-breaking(cervicaldislocation).Soakthemousein70%,wettheabdomenwithwater,lifttheoutskinwithforcepsandcutahorizontalopeningwithscissors,teartheoutsk