文档介绍：HPLCBusinessDeptShimadzuCorporation欢迎大家!?正相色谱(NP-HPLC)?反相色谱(RP-HPLC)?反相离子对色谱(RPIC)?离子交换色谱(IEC)?空间排阻色谱(SEC)?手性化合物分离模式(Chiralseprationmode)HPLCBusinessDeptShimadzuCorporation正相HPLC色谱柱?硅胶型?***基型?氨基型糖类分析?二醇基型蛋白质分析SilicagelSiSi-Si--Si-CH2CH2CH2NH2-Si-CH2CH2CH2OCH(OH)-CH2(OH)ModifiedSiHPLCBusinessDeptShimadzuCorporation相互作用硅胶(polar)氢键Non-polarHPLCBusinessDeptShimadzuCorporation氢键如果样品分子中包含-COOH-NH2-OH氢键作用变强如果样品分子中含有空间阻碍基团氢键作用变弱HPLCBusinessDeptShimadzuCorporation保留时间和氢键OHHOSiOHSiOHStrongWeakHPLCBusinessDeptShimadzuCorporation增强溶剂极性0%2%5%/MeOH1:Dioctylphthalate2:Dibutylphthalate3:Diethylphthalate4:DimethylphthalateSolvent:HexaneHPLCBusinessDeptShimadzuCorporation反相HPLC色谱柱?C18(ODS)type?C8(octyl)type?C4(butyl)type?Phenyltype?TMStype?Cyanotype-Si-C18H35SiNon-polarpropertyHPLCBusinessDeptShimadzuCorporation色谱柱:非极性溶剂:极性水/甲醇/乙***反相色谱