文档介绍：,?技术方法?AUN?,,ZHOUJue-yu,DINGDa-peng,XUQiu-lin,,,SHIRong,MAWen-li(,Guangzhou510515,China;,Guangzhou510800,China)[Abstract]ObjectiveToreportanewmethodoffluorescentlabelingtechniqueinmieroarraystudies:universalprimerU2labeling(UPL).paredoftheUPLwiththatofrandomprimer,restrictiondisplaylabelingmethodandthereversetranscriptioncoupledrandomprimerspikinglabelingmethod(RT-PSL).MethodsInfluenzaviralRNAwaslabeledwithbothUPLandtheconventionalrandomprimerlabelingmethodaswellastwoothermorelaboriouslabelingmethods(RD—directandRD—incorporate),,signal—to—noiseration(SNR),truepositiveratio(11PR),whichmayhavewideapplicationsintheresearchanddevelopmentofthemieroarraytechnology.[Keywords]Universalprimerlabeling(UPL);Influenzavirus;Mieroarray;Labeling[umber]R319[Documentcode]A[ArticleID]0529—1356(2005)03—320一种用于微阵列分析的通用引物U联合标记方法王蜀燕'郑文岭周珏宇'丁大鹏'徐秋林'张海燕'彭翼飞'石嵘'马文丽'(,广州510515;,广州510800)[摘要]目的报告一种新的用于微阵列研究的荧光标记技术:通用引物u联合标记技术(UPL);,与流感病毒寡核苷酸检测芯片杂交后,,信噪比,探针真阳性率和可重复性等方面均高于随机引物逆转录掺人标记法,而与其他两种RD标记方法相当,,在微阵列技术研究方面具有广泛的应用价值.[关键词]通用引物联合标记;流感病毒;微阵列;标记DNAmicmarraysprovidepowerfultoolsfortheglo—balcharacterizationofgeneexpressionandthediagnosisofdiseasesandpathogenicgenes,whiletargetlabelingformicroarrayanalysistypicallyrequiresrelativelylarge【Receiveddate]2005-03-15【Founda