文档介绍：过氧化氢的测定方法Three,catalaseactivity一ultravioletabsorptionmethod[principle]H202hasastrongabsorptionat240nmwavelengths,poseshydrogenperoxide,reducingtheabsorbanee(A240)[]ultravioletspectrophotometer;centrifuge;mortar;volumetricflask250ml1::2ML1:10mltube3;constanttemperaturewaterbath・[reagent]?L~lpH7・8phosphatebuffer(containing1%polyvinylpyrrolidone(L-1H202):??L~1PotassiumPermanganatecalibration).[Methods],withamortar,adding2-3mlat4DEGCpH7・0phosphatebufferprecoolingandasmallamountofquartzsandgrindinghomogenizedinto25mlvolumetricflask,andwashedwithbuffermortarwithseveraltimes,washingliquid,,5DEGCintherefrigeratorstandinglOmin,,supernatantforcatalaseincrudeextracts,,ofwhich2samplesforthetesttube,1forblanktubes,ordancewiththeorder42-2reagents・25Cafterpreheating,,eachtubeimmediatelyafterthe1plustime,andquicklypouredintothequartzcuvette,determinationofabsorbanceof240nm,everylmin1readings,weremeasuredfor34min,ordingtothetypeof42-3・(A240)(U)・Catalaseactivity(-lmin-1)二?Intheformula,Aso--solutiontotakecareisaddedtotheboileddeadenzymeoftheabsorbancevalue;Asl,As2-sampletubelightabsorptionvalue;Vt一一totalextractionvolumeofcrudeenzyme(ML);VI―wasmeasuredwithcrudeenzymeliquidvolume(ML);FW--samplefreshweight(g);-A20perdropwas1enzymeactivityunits(U);T-plushydrogenperoxidetothelastreadingtime(min)・Note:thereisastrongabsorptionofsubstancesunder240nmwhichinterferewiththisexperiment・[thinking]Whatarethefactorsthataffecttheactivityofcatalase?2whatarethebiochemicalprocessesassociatedwithcatalase?References[1],Mukherjee,S,P,Choudhuri,M