文档介绍：口服当归补血汤后阿魏酸在大鼠体内的组织分布作者:王永禄,王丽瑶,陈国广,张东旭,韦萍【摘要】目的研究大鼠灌胃给予当归补血汤后阿魏酸在体内的组织分布特性。方法采用高效液相色谱法;色谱柱为Kromas订C18;流动相为%甲醇乙睛-%磷酸%三乙胺水溶液;检测波长322nm;柱温40°C;流速ml/mino结果各组织中的阿魏酸在10〜400ng范围内与峰面积呈良好的线性关系;回收率平均为%+%;日内和日间的RSD分别%〜%和%〜%之间。阿魏酸在各组织器官中浓度差异较大。结论大鼠灌胃给予当归补血汤后阿魏酸主要在肝脏和肾脏分布,阿魏酸不能通过血脑屏障。【关键词】当归补血汤;阿魏酸;反相-高效液相色谱;组织分布;大鼠Abstract:ObjectiveToinvestigatethetissuedistributionofferulicacid inratsafteroraladministrationofTangkuEiblood-supplementingwasusedColumn:KromasilC18;mobil ephase:%methanol%pthylaminesolution;hwasat322nm;tempervelocitywas/1ineardintheheart,liver,hosphoricacid%triedetectionwavelengtaturewasat40°C;flowrangesofferulicacispleen,-dayandiand%〜 %,respectivelsofferulicacidwereans・ConclusionThemgansofferulicacidieywerelO〜 %土%onaveragnter-dayRSDwere%〜 %・Ferulicacidcannotpassthoughtheblood-barrierKeyw ords:TangkuEIblood -Supplementingdecoction;Ferulicacid; Tissuedistribution;RatsTangkueiBlood-SupplementingDecoctionhasbeenwidelyusedfortonifnganemia,femaleirrandamenorrhoeainthemedicineforhundrestudieshavedemonstlood-SupplementingtlipidperoxidationsofSODandGSH,enhanstoxidation,improvdlessenischemia/raditionalChinesdsofyears・PreviousratedthatTangkueiBDecoctioncaninhibi,maintainactivitiececellabilityagaineimmunefunction,anperfusioninjuryint1].Ferulicacid,ponentsofTangkueiBlood-Supplementin£Decoction[2] ,diminishedtheproductionofH202inhepatocytesandprotectedmembranelipidafterischemia/[ 3 ] ,inhibitingplateletaggregation,inhibitinginflammatoryreaction,anti-oxydationandcleaningfreeradical,,thetissuedistributionofferulicacidinratswasinvesti^atedbyhigh-performanceliquidchromatographyafteroraladministrationofTangkueiblood-